FLOW CYTOMETRIC RAT MICRONUCLEUS ASSAY

化学品在我们的生活质量中扮演着重要的角色，因此，重要的是要确定新化学品的毒性，以便在公开暴露之前能够突出有害物质。微核(MN)试验是评估一种化学物质引起染色体损伤的最广泛使用的系统。这项研究项目将扩大用流式细胞仪(FCM)分析遗传毒素的数据库，评估大鼠外周血(急性或亚慢性处理后)是否适合用于检测遗传毒性化合物造成的损害。由于大鼠骨髓目前被世界各地的监管机构接受，一个主要的研究方向是进一步开发可靠的方法来制备用于FCM分析的骨髓样本。自动化大鼠外周血检测的一个重要方面是，这个物种的脾的行为与人类的相似。因此，这项工作对于我们分析人类血液中是否存在MN的长期目标来说是极其重要的。当我们能够测量人类血液中的MN时，就有可能确定人类意外接触甚至接受化疗的生物监测患者的遗传毒性效应。拟议的商业应用：Litron与Stratagene公司正在开发大鼠外周血和骨髓微核试剂盒。试剂盒将有两种形式：第一种允许研究人员将血液样本送到Litron进行MN分析(微流试剂盒)，以分析MN；第二种允许研究人员在内部分析MN(Micro FlowPlus试剂盒)。制药界反应热烈，并要求开发专门供内部使用的老鼠试剂盒除了试剂盒外，我们预计随着这项先进技术的完善并推向市场，我们的遗传毒理学合同检测将会增加。

Chemicals play an important role in our quality of life, thus it is important to characterize the toxicity of new chemicals so that harmful agents can be highlighted prior to public exposure. The micronucleus (MN) assay is the most widely used system or evaluating a chemical's potential to cause chromosomal damage. This research project will broaden the database of genotoxins analyzed by flow cytometry (FCM), assessing whether rat peripheral blood (after acute or subchronic treatment) is an appropriate compartment for detecting damage caused by genotoxic compounds. Since rat bone marrow is currently accepted by regulatory agencies worldwide, a major line of investigation is to further develop reliable methods for preparing bone marrow samples for FCM analysis. A significant in te rest for automating the rat peripheral blood assay is that the spleen of this species behaves similarly to that of humans. Therefore, this work is extremely important towards our long-term goal of analyzing human blood for the presence of MN. When we are able to measure MN in human blood, it might be possible to determine the genotoxic effects of accidental human exposure and even biomonitor patients receiving chemotherapeutic treatments. PROPOSED COMMERCIAL APPLICATION: Litron, together with the Stratagene Corporation, is developing micronucleus kits for rat peripheral blood and bone marrow. The kits will be in two formats: the first allows researchers to send blood samples to Litron for MN analysis (microFlow kits)to analyze for MN, and the second allows researchers to analyze for MN in-house (micro FlowPlus kits). The pharmaceutical community has responded enthusiastically and has asked for the development of rat kits specifically for their in-house use in addition to the kits, we expect our genetic toxicology contract testing to increase as this advanced technology is refined and brought to the marketplace.