FLOW CYTOMETRIC RAT MICRONUCLEUS ASSAY

化学品在我们的生活质量中起着重要的作用，因此确定新化学品的毒性特征是很重要的，这样在公众接触之前就可以突出有害物质。微核（MN）测定是最广泛使用的系统或评估化学物质的潜力，导致染色体损伤。该研究项目将扩大流式细胞术（FCM）分析基因毒素的数据库，评估大鼠外周血（急性或亚慢性治疗后）是否适合检测基因毒性化合物引起的损伤。由于大鼠骨髓目前已被世界各地的监管机构所接受，因此研究的主要方向是进一步开发可靠的方法来制备用于流式细胞术分析的骨髓样品。自动化大鼠外周血分析的另一个重要意义是，这种动物的脾脏与人类的脾脏行为相似。因此，这项工作对我们分析人类血液中MN的存在的长期目标非常重要。当我们能够测量人体血液中的锰时，就有可能确定人类意外暴露的遗传毒性效应，甚至可以对接受化疗的患者进行生物监测。拟议的商业应用：Litron与Stratagene公司一起，正在开发用于大鼠外周血和骨髓的微核试剂盒。该试剂盒将有两种形式：第一种允许研究人员将血液样本送到Litron进行MN分析（microFlow试剂盒），以分析MN，第二种允许研究人员在内部分析MN （micro FlowPlus试剂盒）。制药界对此反应热烈，并要求开发专门用于内部使用的大鼠试剂盒。除了试剂盒之外，我们还希望随着这项先进技术的完善和推向市场，我们的遗传毒理学合同测试也会增加。

Chemicals play an important role in our quality of life, thus it is important to characterize the toxicity of new chemicals so that harmful agents can be highlighted prior to public exposure. The micronucleus (MN) assay is the most widely used system or evaluating a chemical's potential to cause chromosomal damage. This research project will broaden the database of genotoxins analyzed by flow cytometry (FCM), assessing whether rat peripheral blood (after acute or subchronic treatment) is an appropriate compartment for detecting damage caused by genotoxic compounds. Since rat bone marrow is currently accepted by regulatory agencies worldwide, a major line of investigation is to further develop reliable methods for preparing bone marrow samples for FCM analysis. A significant in te rest for automating the rat peripheral blood assay is that the spleen of this species behaves similarly to that of humans. Therefore, this work is extremely important towards our long-term goal of analyzing human blood for the presence of MN. When we are able to measure MN in human blood, it might be possible to determine the genotoxic effects of accidental human exposure and even biomonitor patients receiving chemotherapeutic treatments. PROPOSED COMMERCIAL APPLICATION: Litron, together with the Stratagene Corporation, is developing micronucleus kits for rat peripheral blood and bone marrow. The kits will be in two formats: the first allows researchers to send blood samples to Litron for MN analysis (microFlow kits)to analyze for MN, and the second allows researchers to analyze for MN in-house (micro FlowPlus kits). The pharmaceutical community has responded enthusiastically and has asked for the development of rat kits specifically for their in-house use in addition to the kits, we expect our genetic toxicology contract testing to increase as this advanced technology is refined and brought to the marketplace.