NON-ISOTOPIC NUCLEIC ACID LABELING REAGENTS

描述（改编自研究者摘要）：一类新的核酸 已经开发了酸标记试剂， 在简单的一步反应中连接各种非同位素标记。 使用这些荧光试剂（荧光素、罗丹明、Cy 3和Cy 5） 和非荧光（生物素、地高辛、二硝基苯酚）可检测标记物可以 以非破坏性的方式附着在DNA或RNA序列上。在I期 研究中，进行了一系列实验，其中标记试剂 进行了：1）优化有效的DNA和RNA标记; 2）测试稳定性 在储存条件和杂交条件下;和3）优化 因为它们能够用于体外杂交反应（即，槽 印迹、Southern印迹和北方印迹）。 在第二阶段的研究中，这些非放射性标记试剂将 经过测试和优化，可用于荧光原位杂交（FISH） 应用.申请人指出，他们的效用是有效的，易于使用， 核酸标记试剂是透明的。他们还指出， 标记将促进探针形成的系统方法， 这是第一次，帮助描述获得所需的关键属性 最高效的原位杂交探针（即，最佳探针长度， 最佳探针长度与标记比率）。 拟议商业应用：不可用

DESCRIPTION (Adapted from the investigator's abstract): A new class of nucleic acid labeling reagents has been developed that facilitates the covalent attachment of a variety of non-isotopic labels in a simple one-step reaction. Using these reagents both fluorescent (fluorescein, rhodamine, Cy3, and Cy5) and non-fluorescent (biotin, digoxin, dinitrophenol) detectable labels can be attached to DNA or RNA sequences in a non-destructive manner. In the Phase I research, a series of experiments were performed in which the labeling reagents were: 1) optimized for efficient DNA and RNA labeling; 2) tested for stability under both storage conditions and hybridization conditions; and 3) optimized for their ability to be used in in vitro hybridization reactions (i.e., slot blots, southern blots, and northern blots). In the Phase II research, these non-radioactive labeling reagents will be tested and optimized for use in fluorescence in situ hybridization (FISH) applications. The applicants state that their utility as efficient, easy to use nucleic acid labeling reagents is clear. They also state that their use in labeling will facilitate a systematic approach to probe formation, that will for the first time, help to delineate the critical attributes needed to obtain the highest efficient in situ hybridization probes (i.e., optimal probe length, optimal probe length to labeling ratio). PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE