CRANIOFACIAL DEVELOPMENTAL MOLECULAR BIOLOGY

颅面发育分子生物学

• 批准号: 6379878
• 负责人: CHARLES F SHULER
• 金额: $ 105.78万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379878
• 关键词: congenital oral /facial /cranial defect; developmental genetics

The Craniofacial Developmental Molecular Biology Program will consist of investigators from the University of Southern California, Schools of Dentistry and Medicine who participate in a multi-disciplinary research team focused on investigations of craniofacial genetics and developmental biology. The faculty collaboration includes investigators from the USC Institute of Genetic Medicine, the USC Comprehensive Cancer Center and the USC Center for Craniofacial Molecular Biology. The overall Program theme will use molecular biology approaches to investigate fundamental mechanisms required for normal development of the craniofacial complex. These findings will be applicable to further the understanding of normal developmental regulation and the molecular etiology of craniofacial birth defects. The Craniofacial Developmental Molecular Biology Program will consist of five Research Projects and one Core Resource. Project 1 (Dr. Yang Chai), "Characterization of Genes Involved in the Specification of Tooth and Meckel's Cartilage Morphogenesis," will characterize the Smad- related intracellular signaling pathway that is activated during mandibular morphogenesis. Project 2 (Dr. Larry Kedes) "Co-Activators and Repressors of Muscle Transcription," will investigate the activity of Twist, p300/CBP and PCAF during the differentiation of muscle cells. Project 3 (Dr. Yi-Hsin Liu) "Genes Involved in Skull Suture Morphogenesis," will examine patters of gene expression unique to the cranial sutures that may be correlated with abnormal suture development resulting in craniosynostosis. Project 4 (Dr. Robert Maxon) "Function of MSX2 and Twist in Calvarial Morphogenesis," will examine the regulation and activation of interacting transcription factors Twist and Msx2 during bone cell commitment and differentiation. Project 5 (Dr. Charles Shuler) " Mechanisms of Epithelial-Mesenchymal Transformation during Palatogenesis," will investigate the molecular mechanism underlying the epithelial-mesenchymal transdifferentiation of the medial edge epithelium during palatal fusion. The activities of the Program will be supported by an Administrative Core that will provide support for purchasing, manuscript preparation, scheduling for seminars and collaborative research planning and financial management. The Program will benefit from the availability of several other established cores at the University of Southern California available to support the research activities of all the Projects. This program will continue a long-term series of collaborations that have provided an excellent scientific environment to advanced the understanding of the molecular mechanisms underling craniofacial development.

颅面发育分子生物学项目将由来自南加州大学、牙科和医学院的研究人员组成，他们参加了一个多学科的研究小组，专注于颅面遗传学和发育生物学的研究。教师合作包括南加州大学遗传医学研究所，南加州大学综合癌症中心和南加州大学颅面分子生物学中心的研究人员。整个项目的主题将使用分子生物学方法来研究颅面复合体正常发育所需的基本机制。这些发现将适用于进一步了解正常的发育调控和颅面出生缺陷的分子病因学。颅面发育分子生物学计划将包括五个研究项目和一个核心资源。项目1（Yang Chai博士），“参与牙齿和Meckel's软骨形态发生规范的基因的表征”，将表征在下颌骨形态发生期间激活的Smad相关细胞内信号通路。项目2（Larry Kedes博士）“肌肉转录的辅助激活剂和抑制剂”将研究肌细胞分化过程中Twist，p300/CBP和PCAF的活性。计画三（刘益欣博士）“颅骨缝形态发生相关基因”将检视颅骨缝特有的基因表现型态，这些基因可能与导致颅缝早闭的异常缝发育有关。项目4（Robert Maxon博士）“MSX 2和Twist在颅骨形态发生中的功能”将研究骨细胞定型和分化过程中相互作用的转录因子Twist和Msx 2的调节和激活。项目5（Charles Shelf博士）“腭发育过程中上皮-间充质转化的机制”将研究腭融合过程中缘上皮细胞-间充质转分化的分子机制。该计划的活动将得到一个行政核心的支持，该核心将为采购、手稿准备、研讨会时间安排以及合作研究规划和财务管理提供支持。该计划将受益于南加州大学其他几个已建立的核心，以支持所有项目的研究活动。该计划将继续长期的一系列合作，提供了一个良好的科学环境，以促进对颅面发育分子机制的理解。