Behavioral Regulation of Neuroendocrine Cells
Behavioral Regulation of Neuroendocrine Cells
批准号:
6330834
负责人:
Emilie F. Rissman
金额:
$9.0万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1996
资助国家:
美国
项目状态:
已结题
起止时间:
1996-09-15 至 2006-04-30
关键词:
androgen receptor aromatase brain metabolism dopamine enzyme activity estrogen receptors estrogens gene induction /repression gene targeting genetically modified animals high performance liquid chromatography hormone regulation /control mechanism immunocytochemistry laboratory mouse male microarray technology microdialysis neuroendocrine system neurogenetics nitric oxide nitric oxide synthase preoptic areas sex behavior
中文摘要
描述:这是一份继续聘用一名研究科学家的建议
发展奖,现在称为独立科学家奖(K02)。在.期间
该奖项的前4年,申请者的研究项目已经扩大
意义重大。申请人发起了一项新的研究,涉及新的
协作和新的技术技能。这个程序利用了
以实验小鼠为模型系统,研究行为神经内分泌学。这个
鼠标提供的优势是其丰富的行为指令
再加上对它的遗传学的高超知识,以及设计
并生产转基因动物。K02奖允许申请者
接受重要的神经和分子生物学方面的新培训
技巧。申请者对性行为的兴趣及其作用
雄激素芳构化为雌激素激活这些行为铅
她开始研究雌激素受体α基因敲除小鼠(ERaKOS)。
她迄今为止的工作表明,尽管性行为和社会
ERaKO男性和女性偏好不足,治疗
多巴胺激动剂阿朴吗啡可以恢复正常行为。这一发现
挑战芳香化雌激素使大脑发育男性化的教条
在胎儿期。进一步评估类固醇受体的作用
在性行为的激活上,PI获得了几个额外的KO
小鼠;包括雌激素受体β基因敲除和TFM小鼠(雄激素
受体基因敲除)。在即将到来的K02授权期内,私人投资公司将审查
使用各种方法进行性行为的分子基础。这些
包括:1)体内微透析以检查神经儿茶酚胺对
一氧化氮与性接触;2)使用高密度DNA阵列
监测基因表达对激素和/或行为相互作用的反应;
3)小鼠分子遗传学培训。K02奖是极其重要的
很有价值,因为它将PI从她的大部分行政和教学工作中解放出来
责任。这使得她可以花大量的时间在
同事的实验室学习新技术并将精力集中在
运行她自己的程序。
英文摘要
DESCRIPTION: This is a proposal for the continuation of a Research Scientist
Development Award, now called an Independent Scientist Award (K02). During the
first 4 years of this award the applicant's research program has expanded
significantly. The applicant initiated a new line of research involving new
collaborations and new technical skills. This program takes advantage of the
laboratory mouse as a model system to study behavioral neuroendocrinology. The
advantages that the mouse offers are the richness of its behavioral repertoire
coupled with a superior knowledge of its genetics, and the ability to design
and produce genetically engineered animals. The K02 award allowed the applicant
to receive significant new training in neuro- and molecular biological
techniques. The applicant's interest in sexual behaviors and the role of
aromatization of androgen to estrogen in the activation of these behaviors lead
her to begin working with the estrogen receptor alpha knockout mouse (ERaKOs).
Her work to date has revealed that although sexual behavior and social
preferences are deficient in ERaKO males and females, treatment with the
dopamine agonist apomorphine can reinstate normal behavior. This finding
challenges the dogma that aromatized estrogens masculinize brain development
during the prenatal period. To further evaluate the role of steroid receptors
in activation of sexual behavior the PI has obtained several additional KO
mice; including the estrogen receptor beta knockout and Tfm mice (androgen
receptor knockouts). During the upcoming K02 grant period the PI will examine
the molecular bases for sexual behavior using a variety of approaches. These
include; 1) in vivo microdialysis to examine neural catecholamine responses to
nitric oxide and sexual encounters; 2) the use of high-density DNA arrays to
monitor gene expression in response to hormones and/or behavioral interactions;
and 3) training in molecular mouse genetics. The K02 award is extremely
valuable since it frees the PI from much of her administrative and teaching
responsibilities. This allows her to spend significant amounts of time in
colleagues' laboratories learning new techniques and focus her energy on
running her own program.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Transgenerational actions of the endocrine disrupting compound Bisphenol A
-
批准号:8694282
-
项目类别:
-
资助金额:$32.83万
-
财政年份:2014
-
负责人:Emilie F. Rissman
-
依托单位:
Transgenerational actions of the endocrine disrupting compound Bisphenol A
-
批准号:9079470
-
项目类别:
-
资助金额:$33.85万
-
财政年份:2014
-
负责人:Emilie F. Rissman
-
依托单位:
Transgenerational actions of the endocrine disrupting compound Bisphenol A
-
批准号:8870353
-
项目类别:
-
资助金额:$33.85万
-
财政年份:2014
-
负责人:Emilie F. Rissman
-
依托单位:
Transgenerational actions of the endocrine disrupting compound Bisphenol A
-
批准号:9230380
-
项目类别:
-
资助金额:$33.85万
-
财政年份:2014
-
负责人:Emilie F. Rissman
-
依托单位:
Genetic and cellular sex differences in cerebellum
-
批准号:8445000
-
项目类别:
-
资助金额:$22.42万
-
财政年份:2012
-
负责人:Emilie F. Rissman
-
依托单位:
Genetic and cellular sex differences in cerebellum
-
批准号:8543778
-
项目类别:
-
资助金额:$19.06万
-
财政年份:2012
-
负责人:Emilie F. Rissman
-
依托单位:
Sex Chromosomes, Epigenetics, and Neurobehavioral Disease
-
批准号:8067079
-
项目类别:
-
资助金额:$37.88万
-
财政年份:2009
-
负责人:Emilie F. Rissman
-
依托单位:
Sex Chromosomes, Epigenetics, and Neurobehavioral Disease
-
批准号:7713062
-
项目类别:
-
资助金额:$37.4万
-
财政年份:2009
-
负责人:Emilie F. Rissman
-
依托单位:
Sex Chromosomes, Epigenetics, and Neurobehavioral Disease
-
批准号:7900841
-
项目类别:
-
资助金额:$38.28万
-
财政年份:2009
-
负责人:Emilie F. Rissman
-
依托单位:
Sex chromosome effects on neural and behavioral differentiation
-
批准号:7989395
-
项目类别:
-
资助金额:$32.3万
-
财政年份:2007
-
负责人:Emilie F. Rissman
-
依托单位:
Sex chromosome effects on neural and behavioral differentiation
-
批准号:7611868
-
项目类别:
-
资助金额:$4.95万
-
财政年份:2007
-
负责人:Emilie F. Rissman
-
依托单位:
Sex chromosome effects on neural and behavioral differentiation
-
批准号:7713543
-
项目类别:
-
资助金额:$36.14万
-
财政年份:2007
-
负责人:Emilie F. Rissman
-
依托单位:
Sex chromosome effects on neural and behavioral differentiation
-
批准号:7211679
-
项目类别:
-
资助金额:$32.98万
-
财政年份:2007
-
负责人:Emilie F. Rissman
-
依托单位:
Sex chromosome effects on neural and behavioral differentiation
-
批准号:7341713
-
项目类别:
-
资助金额:$32.98万
-
财政年份:2007
-
负责人:Emilie F. Rissman
-
依托单位:
Sex chromosome effects on neural and behavioral differentiation
-
批准号:7535182
-
项目类别:
-
资助金额:$41.46万
-
财政年份:2007
-
负责人:Emilie F. Rissman
-
依托单位:
Sex chromosome effects on neural and behavioral differentiation
-
批准号:7454602
-
项目类别:
-
资助金额:$4.78万
-
财政年份:2007
-
负责人:Emilie F. Rissman
-
依托单位:
Reproduction, Nutrition and Behavior
-
批准号:7038264
-
项目类别:
-
资助金额:$33.29万
-
财政年份:2004
-
负责人:Emilie F. Rissman
-
依托单位:
Reproduction, Nutrition and Behavior
-
批准号:6851732
-
项目类别:
-
资助金额:$27.21万
-
财政年份:2004
-
负责人:Emilie F. Rissman
-
依托单位:
Reproduction, Nutrition and Behavior
-
批准号:7215256
-
项目类别:
-
资助金额:$32.32万
-
财政年份:2004
-
负责人:Emilie F. Rissman
-
依托单位:
Reproduction, Nutrition and Behavior
-
批准号:7384493
-
项目类别:
-
资助金额:$32.31万
-
财政年份:2004
-
负责人:Emilie F. Rissman
-
依托单位:
国内基金
海外基金
运动对骨骼肌 Aromatase/17β-estradiol 通路的影响及功能研究
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批准号:19ZR1452900
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项目类别:省市级项目
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资助金额:--
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批准年份:2019
-
负责人:史仍飞
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依托单位: