New Directions in Mutagenized ES Cell Libraries
诱变 ES 细胞文库的新方向
基本信息
- 批准号:6447160
- 负责人:
- 金额:$ 22.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-09-29 至 2004-07-31
- 项目状态:已结题
- 来源:
- 关键词:biotechnology cell line chromosome aberrations electroporation embryonic stem cell expression cloning functional /structural genomics gene expression genetic library genetic manipulation high throughput technology information systems laboratory mouse molecular cloning phenotype polymerase chain reaction proteomics recombinase sequence tagged sites site directed mutagenesis southern blotting technology /technique development transfection /expression vector
项目摘要
DESCRIPTION (provided by applicant): This project will develop new tools to
facilitate high throughput, genome-wide mutagenesis in mouse embryonic Stem
(ES) cells. Functional analysis of the mouse genome is a critical component of
functional genomics, providing information relevant to human gene function and
disease states. Gene trap mutagenesis in ES cells is a proven technology for
generating sequence-tagged insertional mutations in mice. We propose to develop
new gene trap vectors, incorporating a novel integrase-mediated recombination
system. This vector will be used to generate a library of ES clones containing
insertions within a wide range of genes across the genome. This library will be
screened for expression via in vitro expression assays, using differentiation
protocols already developed in the group. Each insertion will also have a RACE
sequence tag associated with it and all information will be incorporated into
an online database for availability to the community. Interesting insertions
can be used to study mutant phenotype after germ line transmission, or can be
used as sites of further gene modification or addition, by means of the
integrase system. A complementary strategy of mutagenesis in ES cells will also
be developed, focusing on in vitro recessive phenotypic screening, combining
chromosome-specific loss of heterozygosity with chemical mutagenesis of ES
cells. Both strategies will be developed first in existing 129 ES cells, but
will be transferred to newly derived C57BL/6 cell lines, as soon as they are
validated, to provide mutations on the B6 'gold standard' background.
描述(由申请人提供):该项目将开发新的工具,以
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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ANDRAS NAGY其他文献
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{{ truncateString('ANDRAS NAGY', 18)}}的其他基金
Generation and characterization of novel and highly specific neruonal subtype tra
新颖且高度特异性的神经亚型 tra 的生成和表征
- 批准号:
7784542 - 财政年份:2008
- 资助金额:
$ 22.5万 - 项目类别:
Generation and characterization of novel and highly specific neruonal subtype tra
新颖且高度特异性的神经亚型 tra 的生成和表征
- 批准号:
7675331 - 财政年份:2008
- 资助金额:
$ 22.5万 - 项目类别:
Generation and characterization of novel and highly specific neruonal subtype tra
新颖且高度特异性的神经亚型 tra 的生成和表征
- 批准号:
8033800 - 财政年份:2008
- 资助金额:
$ 22.5万 - 项目类别:
Contribution to the completion of Comprehensive Mouse Knockout Resource
为完成综合性小鼠基因敲除资源做出贡献
- 批准号:
7285627 - 财政年份:2006
- 资助金额:
$ 22.5万 - 项目类别:
Contribution to the completion of Comprehensive Mouse Knockout Resource
为完成综合性小鼠基因敲除资源做出贡献
- 批准号:
7590791 - 财政年份:2006
- 资助金额:
$ 22.5万 - 项目类别:
Contribution to the completion of Comprehensive Mouse Knockout Resource
为完成综合性小鼠基因敲除资源做出贡献
- 批准号:
7152138 - 财政年份:2006
- 资助金额:
$ 22.5万 - 项目类别:
Contribution to the completion of Comprehensive Mouse Knockout Resource
为完成综合性小鼠基因敲除资源做出贡献
- 批准号:
7392925 - 财政年份:2006
- 资助金额:
$ 22.5万 - 项目类别:
New Directions in Mutagenized ES Cell Libraries
诱变 ES 细胞文库的新方向
- 批准号:
6644197 - 财政年份:2001
- 资助金额:
$ 22.5万 - 项目类别:
New Directions in Mutagenized ES Cell Libraries
诱变 ES 细胞文库的新方向
- 批准号:
6523510 - 财政年份:2001
- 资助金额:
$ 22.5万 - 项目类别:
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