POSTNATAL TARGETING OF STRIATAL OPIOID & NMDA RECEPTORS

产后纹状体阿片类药物的靶向

• 批准号: 6379150
• 负责人: HONG WANG
• 金额: $ 21.19万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379150
• 关键词: NMDA receptors; antireceptor antibody; caudate nucleus; developmental neurobiology; immunocytochemistry; laboratory rat; opioid receptor; receptor binding; receptor expression

DESCRIPTION: (Adapted from the applicant's Description) The targeting of opioid receptors, as well as their coordinated interactions with N-methyl-D-aspartate subtype of glutamate receptors (NMDARs), is crucial for normal brain development and motor functions involving patch-matrix compartments in the mammalian caudate-putamen nucleus (CPN). During the critical developmental plasticity period, mu and delta opioid receptors (MOR, DOR) play either complementary or opposite roles that may in part involve functional interaction with NMDAR. The goal of this application is to test the hypothesis that there are age-related differences in the subcellular localization of MOR and/or DOR, which are correlated with synaptogenesis and parallel those of NMDAR in the CPN. The specific aims in each of the three proposed studies will be pursued by examining the localization of sequence specific antipeptide antisera against MOR, DOR, or NMDAR in the rat CPN during postnatal ontogeny. The results obtained from these studies will complement and extend previous knowledge that age-dependent MOR and DOR subcellular distributions are functionally linked to neuronal maturation; that developing NMDAR is largely involved in activity-dependent synaptic plasticity and has a subcellular distribution that parallels the distribution of opioid receptors; and that prenatal opioid availability differentially controls the ontogeny of neurons containing opioid and/or glutamate receptors. Understanding opioid and NMDA receptor targeting during development in the rat model may provide information leading to new approaches for reactivation of developmental plasticity in human brain.

描述：（根据申请人的描述改编） 阿片受体，以及它们与 谷氨酸受体的N-甲基-D-天冬氨酸亚型（NMDAR），对于 正常的大脑发育和运动功能，涉及斑块基质 在哺乳动物尾壳核（CPN）的隔室。 期间 关键发育可塑性期，μ和δ阿片受体（莫尔， DOR）起补充或相反的作用，可能部分涉及 与NMDAR的功能相互作用。 此应用程序的目标是测试 假设亚细胞中存在与年龄相关的差异， 莫尔和/或DOR的定位，其与突触发生相关， 与CPN中的NMDAR相似。 三个方面的具体目标 建议的研究将通过检查序列的定位来进行 大鼠CPN中抗莫尔、DOR或NMDAR的特异性抗肽抗血清， 出生后个体发育 从这些研究中获得的结果将补充 并扩展了以前的知识，即年龄依赖性莫尔和DOR亚细胞 分布在功能上与神经元成熟有关;发育中的 NMDAR在很大程度上参与活性依赖性突触可塑性，并具有 与阿片受体分布平行的亚细胞分布; 产前阿片类药物的可用性差异控制个体发育， 神经元含有阿片和/或谷氨酸受体。 了解阿片类药物 和NMDA受体靶向在大鼠模型的发展过程中， 信息导致新的方法来重新激活发展 人类大脑的可塑性