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POSTNATAL TARGETING OF STRIATAL OPIOID & NMDA RECEPTORS

产后纹状体阿片类药物的靶向

基本信息

批准号：
6798712
负责人：
HONG WANG
金额：
$ 21.19万
依托单位：
WEILL MEDICAL COLL OF CORNELL UNIV
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-09-30 至 2006-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6798712
关键词：
NMDA receptors antireceptor antibody caudate nucleus developmental neurobiology immunocytochemistry laboratory rat opioid receptor receptor binding receptor expression

项目摘要

DESCRIPTION: (Adapted from the applicant's Description) The targeting of opioid receptors, as well as their coordinated interactions with N-methyl-D-aspartate subtype of glutamate receptors (NMDARs), is crucial for normal brain development and motor functions involving patch-matrix compartments in the mammalian caudate-putamen nucleus (CPN). During the critical developmental plasticity period, mu and delta opioid receptors (MOR, DOR) play either complementary or opposite roles that may in part involve functional interaction with NMDAR. The goal of this application is to test the hypothesis that there are age-related differences in the subcellular localization of MOR and/or DOR, which are correlated with synaptogenesis and parallel those of NMDAR in the CPN. The specific aims in each of the three proposed studies will be pursued by examining the localization of sequence specific antipeptide antisera against MOR, DOR, or NMDAR in the rat CPN during postnatal ontogeny. The results obtained from these studies will complement and extend previous knowledge that age-dependent MOR and DOR subcellular distributions are functionally linked to neuronal maturation; that developing NMDAR is largely involved in activity-dependent synaptic plasticity and has a subcellular distribution that parallels the distribution of opioid receptors; and that prenatal opioid availability differentially controls the ontogeny of neurons containing opioid and/or glutamate receptors. Understanding opioid and NMDA receptor targeting during development in the rat model may provide information leading to new approaches for reactivation of developmental plasticity in human brain.

描述：（改编自申请人的描述）阿片受体及其与阿片受体的协调相互作用谷氨酸受体 (NMDAR) 的 N-甲基-D-天冬氨酸亚型对于正常的大脑发育和运动功能涉及补片矩阵哺乳动物尾状壳核 (CPN) 中的区室。期间关键发育可塑期、mu 和 delta 阿片受体（MOR、 DOR）发挥互补或相反的作用，可能部分涉及与 NMDAR 的功能相互作用。该应用程序的目标是测试亚细胞中存在与年龄相关的差异的假设 MOR 和/或 DOR 的定位，与突触发生相关与 CPN 中的 NMDAR 类似。三者各自的具体目标拟议的研究将通过检查序列的定位来进行大鼠 CPN 期间针对 MOR、DOR 或 NMDAR 的特异性抗肽抗血清出生后个体发育。从这些研究中获得的结果将补充并扩展了先前的知识，即年龄依赖性 MOR 和 DOR 亚细胞分布在功能上与神经元成熟相关；那个发展中的 NMDAR 主要参与活动依赖性突触可塑性，并具有与阿片受体分布平行的亚细胞分布；产前阿片类药物的可用性差异性地控制着个体发育含有阿片受体和/或谷氨酸受体的神经元。了解阿片类药物大鼠模型发育过程中 NMDA 受体靶向可能提供信息导致重新激活发育的新方法人脑的可塑性。