STREPTOCOCCUS PNEUMONIAE ADHERENCE AND OTITIS MEDIA PATH

肺炎链球菌依从性和中耳炎媒介路径

• 批准号: 6379373
• 负责人: THOMAS F DEMARIA
• 金额: $ 27.64万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379373
• 关键词: Adenoviridae; Streptococcus infection; Streptococcus pneumoniae; Streptococcus vaccine; bacteria infection mechanism; bacterial antigens; carbohydrate analog; carbohydrate receptor; cell adhesion; chinchilla; disease /disorder model; enzyme inhibitors; exo alpha sialidase; host organism interaction; influenzavirus A; microorganism interaction; nasopharynx; otitis media; pathologic process; respiratory epithelium; trachea; vaccine development

DESCRIPTION: (Adapted from the Investigator's Abstract) Otitis media (middle ear inflammation) ranks first among the most common diagnoses requiring a physician's office visit and recent estimates indicate that virtually all children (99%) will experience a least one episode of otitis media (OM) by age 2. The disease progresses in many children to recurrent infections and chronic inflammation, often with complications and sequalae that include persistent hearing loss nd communication disorders. Streptococcus pneumoniae (Spn), is one of the foremost human pathogens and is the primary nasopharynx, the initial event in the induction of OM and the mechanisms which effect the transition for a colonized state to invasion of the middle ear and the induction of the disease state by Spn are not known. The long term objectives of this proposal are to delineate the pathogenic mechanisms involved in Spn adherence, colonization, and invasion of the middle ear and to develop a scientific rationale for the design of novel diagnostic and prevention strategies. The specific aims of this proposal are: 1) To continue to define the role of Spn neuraminidase in the pathogenesis of OM and to assess whether it is a protective antigen and a potential protein-based vaccine candidate, and whether neuraminidase inhibitors are effective in the prevention of Spn nasopharyngeal colonization of OM. 2) To assess the efficacy of lacto-N-neotetraose, a Spn carbohydrate receptor analog, as an anti-infective for nasopharyngeal colonization. 3) To continue to define the mechanisms whereby influenza A virus affects Spn adherence, colonization, and OM. These aims are designed to delineate the initial interaction, adherence, and colonization which represents the first in a series of steps that culminates in otitis media. These studies may suggest avenues for blocking interaction of Spn with host cells either by immunization or direct intervention blockade with isolated receptor moieties or bacterial adhesin components.

描述：（改编自调查员的摘要）中耳炎（中间 耳朵发炎）在需要的最常见诊断中排名第一 医师的办公访问和最近的估计表明，几乎所有 儿童（99％）将以年龄的年龄至少经历一次中耳炎媒体（OM） 2。许多儿童的疾病发展为复发感染和慢性 炎症，通常带有并发症和序列，包括持续 听力损失和沟通障碍。肺炎链球菌（SPN）是一个 最重要的人类病原体，是主要的鼻咽，初始 OM诱导的事件和影响过渡的机制 一个殖民的状态，以入侵中耳和诱导 SPN的疾病状态尚不清楚。该提议的长期目标 要描述与SPN依从性有关的致病机制， 殖民化，中耳入侵并发展科学 设计新型诊断和预防策略的理由。这 该提案的具体目的是：1）继续定义SPN的作用 神经氨酸酶在OM的发病机理中，并评估它是否是一个 保护性抗原和潜在的基于蛋白质的疫苗候选者，以及是否是否 神经氨酸酶抑制剂可有效预防SPN鼻咽 OM的殖民化。 2）评估SPN乳酸-N-诺糖的功效 碳水化合物受体类似物，作为鼻咽的抗感染 殖民化。 3）继续定义流感病毒的机制 影响SPN的依从性，定殖和OM。这些目标旨在 描述代表的初始相互作用，依从性和定殖 在一系列步骤中的第一步，最终导致中耳炎。这些研究 可能建议通过 通过孤立受体部分或 细菌粘附成分。