MECHANISM-BASED IN VITRO SCREENING OF CHEMOPREVENTIVE AG

目的(A)以自发永生化的Li-Fraumeni乳腺上皮细胞为研究对象，评价化学预防药物对其永生化过程中相关标志物的影响。在目标(B)中，建立了基于主要可遗传癌症综合征患者的hTERT永生化皮肤成纤维细胞的潜在的化学预防药物的筛选系统。这些细胞进一步与已知的参与癌症进展的遗传因素进行转导。确定了化学预防药物对这些细胞的影响。

In objective (A) Li-Fraumeni breast epithelial cells which spontaneously immortalize are used to to evaluate the efficacy of chemopreventive agents to inhibit the spontaneous immortalization of these cells, including their effect on relevant markers during the process of immortalization. In objective (B) Potential screen systems for chemopreventive agents are established that are based on hTERT-immortalized skin fibroblasts from patients with dominantly heritable cancer syndromes. These cells are further transduced with known genetic elements which are involved in cancer progression. The effects of chemopreventive agents on these cells are determined.