OVARIAN STIMULATION & OOCYTE COMPETENCE IN PRENATALLY ANDROGENIZED FEMALE RHESUS

为了确定是否产前雄激素过多， 雌性恒河猴的早期妊娠导致 作为研究的一部分， 调查高雄激素血症患者流产增加的原因 多囊卵巢综合征（PCOS）的发病原因 结果产前2例 具有排卵月经周期的雄激素化恒河猴， 用重组人FSH刺激以用于体内收集 成熟卵母细胞 通过腹腔镜抽吸卵母细胞，并 在第一极体挤出后进行体外授精。 虽然从两只猴子中回收了正常数量的卵母细胞， 获得的成熟（中期II）卵母细胞的比例为 与我们的长期平均水平相比，相对较低（约31%） 正常雌性恒河猴（76%）。 在一个产前 雄激素化猴（#79135），没有卵母细胞完成 hCG后41小时前成熟，而正常对照组为34小时。 雌性猴子，没有受精，尽管有许多能动的精子 12小时后附着于透明蜱上。 在另一只产前雄激素化的猴子（#79169）中，尽管 成熟是相当低的，受精和 发育到胚泡阶段的比例与对照组相似（75%和 65%）。 这些非常初步的数据表明，卵母细胞 从产前雄激素化的猴子，可能会损害他们的能力， 成熟和施肥。 未来的方向我们计划探索 从产前雄激素化的猴子身上采集的卵母细胞 在卵巢刺激后， 并在体外进行完整的植入前发育， 新的见解，流产的高发病率经历了 PCOS的高雄激素女性 关键词卵母细胞，卵巢，多囊 卵巢综合征，雄激素过多，流产 RR 00167，UW研究生院研究委员会，UW医学院 委员会

OBJECTIVES To determine whether prenatal androgen excess during early gestation in female rhesus monkeys contributes to impaired oocyte developmental competency in adulthood, as part of a study Investigating the origins of increased miscarriage in hyperandrogenic women with polycystic ovarian syndrome (PCOS). RESULTS Two prenatally androgenized rhesus monkeys with ovulatory menstrual cycles were stimulated with recombinant human FSH for collection of in vivo matured oocytes. Oocytes were aspirated laparoscopically, and inseminated in vitro following extrusion of the first polar body. Although normal numbers of oocytes were recovered from both monkeys, the proportion of mature (metaphase II) oocytes that were obtained was relatively low (approximately 31%), compared to our long-term average for normal female rhesus monkeys (76%). In one of the prenatally androgenized monkeys (#79135), none of the oocytes completed maturation before 41 hours post hCG, compared to 34 hours for normal female monkeys, and none fertilized, although numerous motile sperm were attached to the zona pellucidae 12 hours after inseminination. In the other prenatally androgenized monkey (#79169), although maturation was quite low, both the incidence of fertilization and development to the blastocyst stage were similar to controls (75% and 65%, respectively). These very preliminary data suggest that oocytes from prenatally androgenized monkeys may be impaired in their ability to mature and to fertilize. FUTURE DIRECTIONS We plan to explore whether oocytes collected from prenatally androgenized monkeys following ovarian stimulation are impaired in their capacity to mature and undergo complete preimplantation development in vitro, to provide novel insight into the high incidence of miscarriage experienced by hyperandrogenic women with PCOS. KEY WORDS oocyte, ovary, polycystic ovarian syndrome, androgen excess, miscarriage FUNDING NIH grant RR00167, UW Graduate School Research Committee, UW Medical School Committee