PARACRINE DYSREGULATION OF OOCYTE COMPETENCE IN POLYCYSTIC OVARY SYNDROME

多囊卵巢综合征中卵母细胞能力的旁分泌失调

• 批准号: 7716415
• 负责人: Daniel A Dumesic
• 金额: $ 4.1万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-23 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7716415
• 关键词: Accounting; Affect; Age; Androgens; Animals; Anovulation; Biological Assay; Biological Markers; Competence; Computer Retrieval of Information on Scientific Projects Database; Development; Embryonic Development; Endocrine; Exhibits; Female; Fertility; Fertilization in Vitro; Follicle Stimulating Hormone; Follicular Fluid; Funding; Gonadotropins; Grant; Human; Hyperandrogenism; Infertility; Institution; Insulin Resistance; Investigation; Macaca mulatta; Modeling; Monkeys; Oocytes; Ovarian; Pathology; Physiological; Polycystic Ovary Syndrome; Pregnancy Outcome; Pregnancy loss; Protocols documentation; Reproductive Health; Research; Research Personnel; Resources; Services; Source; United States National Institutes of Health; Woman; egg; experience; hormone therapy; improved; oocyte maturation; paracrine; psychologic; reproductive

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To further develop the prenatally androgenized (PA) female rhesus monkey as a model for PCOS. Polycystic ovary syndrome (PCOS) in women is characterized by anovulation, LH hypersecretion, hyperandrogenism and insulin resistance. As the most common female endocrine pathology, affecting 4-7% of reproductive-aged women, and as a frequent cause of infertility, accounting for 75% of anovulation, PCOS has staggering adverse physiological, psychological and financial consequences for women's reproductive health. During gonadotropin stimulation for in vitro fertilization (IVF), PCOS women experience decreased fecundity and increased pregnancy loss. Since experimental investigation of egg and embryo development in humans is limited by ethical constraints, we have developed the prenatally androgenized (PA) female rhesus monkey as a model for PCOS. In an on-going study, PA female monkeys undergoing follicle stimulating hormone (FSH) therapy for IVF exhibit androgen excess in the follicular fluid of stimulated follicles, typical of PCOS women undergoing similar fertility treatment, while also requiring more FSH treatment to produce mature oocytes compared to normal female monkeys. Follicular androgen excess may perturb the development of mature oocytes, and the increased requirement for FSH treatment suggests reduced ovarian reserve of follicles. Both abnormalities may compromise oocyte maturation. The long-term objectives of this proposal are to define molecular markers of egg developmental competence that enhance IVF pregnancy outcome by improving embryo development, while minimizing pregnancy loss in women with PCOS. This research used WNPRC Animal Services, Assay Services and Central Protocol Implementation unit. Funding ended Aug. 31, 2007.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 为了进一步发展产前雄激素化（PA）雌性恒河猴作为PCOS的模型。 女性的多囊卵巢综合征（PCOS）的特征在于缺乏，LH分泌，超雄激素和胰岛素抵抗。作为最常见的女性内分泌病理学，影响了4-7％的生殖老年妇女，并且经常导致不育的原因，占主体的75％，对女性生殖健康产生了惊人的不良生理，心理和财务后果。在促性腺激素刺激体外受精（IVF）期间，PCOS妇女的经历降低了繁殖力和妊娠丧失。由于人类卵和胚胎发育的实验研究受伦理限制的限制，因此我们开发了产前雄激素化（PA）雌性恒河猴作为PCOS的模型。在一项正在进行的研究中，正在接受卵泡激素（FSH）治疗的PA雌性猴子在刺激的卵泡的卵泡液中表现出雄激素过量，这是典型的PCOS妇女接受类似生育能力的PCOS妇女，同时还需要与正常雌性雌性雌性相比，需要更多的FSH治疗来产生成熟的卵母细胞。卵泡雄激素过量可能会扰动成熟的卵母细胞的发展，而对FSH处理的需求增加表明卵泡的卵巢储备减少。两种异常可能会损害卵母细胞的成熟。该提案的长期目标是定义卵发育能力的分子标记，从而通过改善胚胎发育来增强IVF妊娠结局，同时最大程度地减少PCOS女性的妊娠损失。这项研究使用了WNPRC动物服务，测定服务和中央协议实施单元。 资金于2007年8月31日结束。