PARACRINE DYSREGULATION OF OOCYTE COMPETENCE IN POLYCYSTIC OVARY SYNDROME

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 进一步发展产前雄激素化（PA）雌性恒河猴作为PCOS模型。 多囊卵巢综合征（PCOS）是一种以无排卵、LH分泌过多、高雄激素血症和胰岛素抵抗为特征的疾病。作为最常见的女性内分泌疾病，影响4-7%的育龄妇女，并作为不孕症的常见原因，占不排卵的75%，PCOS对妇女的生殖健康具有惊人的不良生理，心理和经济后果。在促性腺激素刺激体外受精（IVF）期间，PCOS妇女的生育力下降，妊娠丢失增加。由于人类卵子和胚胎发育的实验研究受到伦理约束的限制，我们开发了产前雄激素化（PA）雌性恒河猴作为PCOS的模型。在一项正在进行的研究中，接受卵泡刺激素（FSH）治疗进行IVF的PA雌猴在刺激卵泡的卵泡液中表现出雄激素过量，这是接受类似生育治疗的PCOS女性的典型特征，同时与正常雌猴相比，还需要更多的FSH治疗来产生成熟的卵母细胞。卵泡雄激素过多可能会干扰成熟卵母细胞的发育，对FSH治疗的需求增加表明卵泡的卵巢储备减少。这两种异常都可能影响卵母细胞的成熟。该提案的长期目标是定义卵子发育能力的分子标志物，通过改善胚胎发育来提高IVF妊娠结局，同时最大限度地减少PCOS女性的妊娠损失。本研究使用了WNPRC动物服务、分析服务和中央方案实施单位。 资金于2007年8月31日结束。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To further develop the prenatally androgenized (PA) female rhesus monkey as a model for PCOS. Polycystic ovary syndrome (PCOS) in women is characterized by anovulation, LH hypersecretion, hyperandrogenism and insulin resistance. As the most common female endocrine pathology, affecting 4-7% of reproductive-aged women, and as a frequent cause of infertility, accounting for 75% of anovulation, PCOS has staggering adverse physiological, psychological and financial consequences for women's reproductive health. During gonadotropin stimulation for in vitro fertilization (IVF), PCOS women experience decreased fecundity and increased pregnancy loss. Since experimental investigation of egg and embryo development in humans is limited by ethical constraints, we have developed the prenatally androgenized (PA) female rhesus monkey as a model for PCOS. In an on-going study, PA female monkeys undergoing follicle stimulating hormone (FSH) therapy for IVF exhibit androgen excess in the follicular fluid of stimulated follicles, typical of PCOS women undergoing similar fertility treatment, while also requiring more FSH treatment to produce mature oocytes compared to normal female monkeys. Follicular androgen excess may perturb the development of mature oocytes, and the increased requirement for FSH treatment suggests reduced ovarian reserve of follicles. Both abnormalities may compromise oocyte maturation. The long-term objectives of this proposal are to define molecular markers of egg developmental competence that enhance IVF pregnancy outcome by improving embryo development, while minimizing pregnancy loss in women with PCOS. This research used WNPRC Animal Services, Assay Services and Central Protocol Implementation unit. Funding ended Aug. 31, 2007.