AUTOFIT 1 2, UPDATED SOFTWARE FOR XAS GLOBAL MAPPING

AUTOFIT 1 2，XAS 全球地图更新软件

• 批准号: 6345120
• 负责人: MARK R CHANCE
• 金额: $ 2.8万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6345120
• 关键词: bacteria; biological products; biomedical resource; enzymes; proteins; spectrometry

We have also completed the first characterization of the active site of NiSOD from Streptomyces seoulensis. This study reveals that Ni site is bound to thiolate donors, like other Ni redox enzymes but unlike any previously characterized SOD. Examination of oxidized and reduced samples reveal a coordination number change (5 -> 4) and a shift in the edge energy appropriate for a one-electron process. There is also evidence that the active site consists of two Ni centers, one of which is not redox active in analogy with the CuZnSOD, and therefore must be composed of two subunits in the tetrameric enzyme.

我们也完成了第一个活性的表征