STRUCTURAL AND FUNCTIONAL WATER DYNAMICS IN RHODOPSIN ACTIVATION FROM PICOSECON

PICOSECON 视紫红质激活中的结构和功能水动力学

• 批准号: 8364358
• 负责人: MARK R CHANCE
• 金额: $ 0.11万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364358
• 关键词: Biological Models; Biomedical Research; Blood Glucose; Blood Pressure; Cell Surface Receptors; Communication; DNA Sequence Rearrangement; Drug Delivery Systems; Family; Funding; G-Protein-Coupled Receptors; Grant; Heart Rate; High Performance Computing; Human body; Marketing; Mass Spectrum Analysis; National Center for Research Resources; Principal Investigator; Process; Regulation; Research; Research Infrastructure; Resources; Rhodopsin; Role; Source; Structure; Taste Perception; Technology; United States National Institutes of Health; Vision; Water; base; computerized data processing; cost; glucose metabolism; interest; novel; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. G-protein coupled receptors (GPCR) are the largest family of cell surface receptors and are responsible for the regulation of a myriad of processes in the human body including regulation of heart rate, blood pressure and glucose metabolism as well as the senses of sight and taste. Because of their central roles in cellular communication, GPCRs have been of fundamental interest in modern pharmacological research; e.g. approximately 50% of marketed drugs target GPCRs. In this proposal we will use the resources of Anton to explore the structure and functional consequences of water rearrangements in the signaling processes of GRPCs, using rhodopsin as our model system. This will help drive experiments to detect specific water rearrangements in GPCRs using novel mass spectrometry based technologies.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 G蛋白偶联受体（GPCR）是最大的细胞表面受体家族，负责调节人体内的无数过程，包括调节心率、血压和葡萄糖代谢以及视觉和味觉。由于其在细胞通讯中的核心作用，GPCR 引起了现代药理学研究的根本兴趣。例如大约 50% 的上市药物以 GPCR 为靶点。在本提案中，我们将利用 Anton 的资源，以视紫红质作为我们的模型系统，探索 GRPC 信号传导过程中水重排的结构和功能后果。这将有助于推动使用基于质谱的新型技术检测 GPCR 中特定水重排的实验。