STRUCTURAL AND FUNCTIONAL WATER DYNAMICS IN RHODOPSIN ACTIVATION FROM PICOSECON

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 G蛋白偶联受体（GPCR）是细胞表面受体的最大家族，并且负责调节人体中的无数过程，包括调节心率、血压和葡萄糖代谢以及视觉和味觉。由于其在细胞通讯中的核心作用，GPCR在现代药理学研究中具有根本意义;例如，约50%的市售药物靶向GPCR。在这个提议中，我们将利用Anton的资源，以视紫红质为模型系统，探索GRPC信号转导过程中水重排的结构和功能后果。这将有助于推动实验，使用基于质谱的新技术检测GPCR中的特定水重排。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. G-protein coupled receptors (GPCR) are the largest family of cell surface receptors and are responsible for the regulation of a myriad of processes in the human body including regulation of heart rate, blood pressure and glucose metabolism as well as the senses of sight and taste. Because of their central roles in cellular communication, GPCRs have been of fundamental interest in modern pharmacological research; e.g. approximately 50% of marketed drugs target GPCRs. In this proposal we will use the resources of Anton to explore the structure and functional consequences of water rearrangements in the signaling processes of GRPCs, using rhodopsin as our model system. This will help drive experiments to detect specific water rearrangements in GPCRs using novel mass spectrometry based technologies.