3D STRUCT OF ENZYMES INTERACTING W/ NUCLEOTIDES: ESTROGEN BINDING

酶与核苷酸相互作用的 3D 结构：雌激素结合

• 批准号: 6339144
• 负责人: SHENG-XIANG LIN
• 金额: $ 0.99万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2001-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6339144
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

Data were collected at MIT from crystals of CHK SH2 and a data set complete to 3Angstroems was obtained. Molecular replacement was attempted but a solution was not found. The most logical path to take now would have been to search for heavy atom derivatives and collect data. Unfortunately at the time our Laboratory was not equipped with an X-ray facility and we were unable to secure more time at an external establishment. We therefore applied for time at F2 in order to get MAD phases or as a contingency to collect heavy atom data. In the limited time we had at CHESS (around 2.5 days or less) we were unable to find suitable cryo-conditions for the crystals and had to resort to collecting data from potential heavy-atom derivatives. Four potential heavy atom data sets were collected at CHESS F2 and we are in the process of using this data to obtain starting phases for the SH2 domain. Small crystals of the SH2 domain complexed with the receptor peptide ERB-B2 which is expressed in mammalian breast cancer cells have also been grown recently and efforts are being directed to increase the size of these crystals. The crystal structure of the complex would be of fundamental importance in understanding the role of CHK in signalling in breast cancer.

数据是在麻省理工学院从CHK SH2的晶体和一个数据集收集的 获得完整的3埃。分子置换是 已尝试，但未找到解决方案。最合乎逻辑的道路 现在应该是搜索重原子的衍生品并收集 数据。不幸的是，当时我们的实验室没有配备 一个X光设备，我们无法在一个 外部机构。因此我们按顺序申请了F2的时间 以获得MAD相，或作为收集重原子数据的应急措施。在……里面 我们下棋的有限时间(大约2.5天或更少)是 无法为晶体找到合适的低温条件，不得不 求助于从潜在的重原子衍生品中收集数据。四 潜在的重原子数据集是在国际象棋F2收集的，我们正在 在使用此数据获取 Sh2结构域。SH2结构域的小晶体与 受体多肽ERB-B2在哺乳动物乳腺癌中的表达 细胞最近也得到了培养，目前正在努力 增大这些晶体的大小。该化合物的晶体结构 复杂性对于理解这一角色将是至关重要的 CHK在乳腺癌的信号传递中的作用。