3D STRUCT OF ENZYMES INTERACTING W/ NUCLEOTIDES: ESTROGEN BINDING

在MIT从CHK SH 2的晶体收集数据，并且数据集 获得了完整的3Angstroems。 分子置换是 尝试但未找到解决方案。最符合逻辑的方法是 现在的任务是寻找重原子衍生物， 数据 不幸的是，当时我们的实验室没有配备 一个X光设施，我们无法获得更多的时间在一个 外部机构。 因此，我们申请了F2的时间，以便 以获得MAD阶段或作为收集重原子数据的应急措施。 在 我们在国际象棋有限的时间（大约2.5天或更少），我们 无法找到适合晶体的低温条件， 从潜在的重原子衍生物中收集数据。 四 在CHESS F2上收集了潜在的重原子数据集， 在使用这些数据来获得初始阶段的过程中， SH 2结构域。 SH 2结构域的小晶体与 在哺乳动物乳腺癌中表达的受体肽ERB-B2 最近也培养了细胞，并且正在努力 增加这些晶体的尺寸。 的晶体结构 复杂性对于理解 CHK在乳腺癌信号传导中的作用。

Data were collected at MIT from crystals of CHK SH2 and a data set complete to 3Angstroems was obtained. Molecular replacement was attempted but a solution was not found. The most logical path to take now would have been to search for heavy atom derivatives and collect data. Unfortunately at the time our Laboratory was not equipped with an X-ray facility and we were unable to secure more time at an external establishment. We therefore applied for time at F2 in order to get MAD phases or as a contingency to collect heavy atom data. In the limited time we had at CHESS (around 2.5 days or less) we were unable to find suitable cryo-conditions for the crystals and had to resort to collecting data from potential heavy-atom derivatives. Four potential heavy atom data sets were collected at CHESS F2 and we are in the process of using this data to obtain starting phases for the SH2 domain. Small crystals of the SH2 domain complexed with the receptor peptide ERB-B2 which is expressed in mammalian breast cancer cells have also been grown recently and efforts are being directed to increase the size of these crystals. The crystal structure of the complex would be of fundamental importance in understanding the role of CHK in signalling in breast cancer.