DNA POLYMERASE FROM FISSION YEAST

来自裂殖酵母的 DNA 聚合酶

• 批准号: 6308851
• 负责人: TERESA S WANG
• 金额: $ 0.99万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308851
• 关键词: biomedical resource; genome; microorganism; nucleic acids; proteins; spectrometry; structural biology

DNA polymerase a is isolated as a protein-complex by affinity chromatography. The subunits are then separated by SDS gel electrophoresis. The proteins are in-gel digested. The peptides are separated by reversed phase HPLC. Molecular weight and sequence information obtained by MALDI, MALDI-PSD and MALDI-CID are then used to identify the protein in the S. ponbe database or for oligonucleotide probes for PCR and subsequent DNA sequencing experiments.

DNA聚合酶a作为蛋白质复合物通过亲和层析分离。 层析 然后通过SDS凝胶将亚基分离 电泳 蛋白质在凝胶内消化。 所述肽 通过反相HPLC分离。 分子量和序列 然后使用通过MALDI、MALDI-PSD和MALDI-CID获得的信息 以鉴定S. ponbe数据库或用于 用于PCR和随后的DNA测序的寡核苷酸探针 实验