PURINE METABOLISM IN SCHISTOMA MANSONI

曼索尼血吸虫中的嘌呤代谢

• 批准号: 6308896
• 负责人: Ching Chung WANG
• 金额: $ 0.99万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308896
• 关键词: biomedical resource; drug /agent; enzymes; infection; parasitism; spectrometry; structural biology

S. mansoni hypoxanthine-guanine phosphoribosyltransferase (HGPRTase) was identified as one of the pivotal enzymes in the purine salvage by the organism. One of our research goals is to verify the N-terminus of the HGPRTase and use the sequence for design of a vector for synthesis of the enzyme in bacteria. Conventional sequencing indicated that the N-terminus may be blocked, necessitating the use of alternate approaches, such as mass spectrometry.

S. 曼氏次黄嘌呤鸟嘌呤磷酸核糖基转移酶 HGPRT酶是嘌呤代谢的关键酶之一， 有机体的救助。 我们的研究目标之一是验证 HGPRT酶的N-末端，并使用该序列设计载体 用于细菌中酶的合成。 常规测序 表明N-末端可能被封闭，需要使用 替代方法，例如质谱法。