PERINUCLEOLAR COMPARTMENT AND CANCER

核仁周室（PNC）是一种独特的核结构， 形成与恶性表型相关， 细胞和人类癌症。我们的长期目标是了解 PNC的功能及其在发展和 恶性表型的维持。我们将开始， 三个问题。1)发生的功能事件有哪些 PNC？我们最近表明，PNC结合标记 在很短的时间内，核苷酸，这表明它的参与， RNA代谢。我们会澄清成立法团是否代表 转录，或RNA的转录后修饰，或 两者的组合，通过分析其中标记的 通过确定PNC是否与 与染色体和/或特定基因，并通过检查的存在， 转录因子和RNA加工因子。 2)PNC的形成机制是什么？我们有 发现PNC的完整性受转录因子的影响， 细胞的状态。我们将通过实验改变rRNA的水平 和/或一些pol III基因的表达，从而检查 两种聚合酶活性之间的潜在协调， 他们对PNC流行的影响。3)什么是分子 PNC的成员？我们将分离并鉴定 蛋白质和RNA是PNC的一部分，从而产生更多的 我们将利用这些信息来寻找 国家民警的组成及其职能。的结果予以 实验将提供一个重要的基础，以解决 PNC在恶性细胞中的意义。

The perinucleolar compartment (PNC) is a unique nuclear structure whose formation is correlated with the malignant phenotype both in cultured cells and in human cancer. Our long term objective is to understand the function of the PNC and its significance in the development and maintenance of the malignant phenotype. We will begin by addressing three problems. 1) What are the functional events that take place in the PNC? We have recently shown that the PNC incorporates labeled nucleotides within a short period of time, suggesting its involvement in RNA metabolism. We will clarify whether the incorporation represents transcription, or the post-transcriptional modification of RNA, or a combination of both, by analyzing the sequences where the labeled nucleotides are inserted, by determining whether the PNC is associated with chromosome and/or specific genes, and by examining the presence of transcription factors and RNA processing factors in the PNC. 2) What are the mechanisms that induce the formation of the PNC? We have found that the integrity of the PNC is influenced by the transcriptional state of the cells. We will experimentally change the level of rRNA and/or some of the pol III gene expression, and thereby examine the potential coordination between the activities of the two polymerases and their effect on the prevalence of the PNC. 3) What are the molecular constituents of the PNC? We will isolate and identify additional proteins and RNAs that are part of the PNC, thus generating more information with which we will search for factors that are responsible for the formation of the PNC and its function. The results of these experiments will provide an important foundation to address the significance of the PNC in malignant cells.