MOUSE MODEL OF RETARDATION IN DOWNS SYNDROME
唐氏综合症小鼠发育迟缓模型
基本信息
- 批准号:6327097
- 负责人:
- 金额:$ 22.57万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-04-01 至 2004-02-28
- 项目状态:已结题
- 来源:
- 关键词:AMPA receptors Downs syndrome NMDA receptors biological models biological signal transduction cAMP response element binding protein computer data analysis cyclic AMP electrophysiology gene expression hippocampus laboratory mouse long term potentiation mental retardation model design /development molecular pathology neuropathology neurophysiology phosphorylation protein kinase A protein kinase C sectioning voltage /patch clamp voltage gated channel western blottings
项目摘要
DESCRIPTION (Provided by applicant): Down syndrome (DS) or trisomy 21 (Ts21) is
caused by the presence of three copies of chromosome 21, and is the most
frequent genetic cause of mental retardation. The Ts65Dn trisomic mouse has
recently been developed and is trisomic only for the segment of murine
chromosome 16 that is homologous to the segment of human chromosome 21 thought
to contribute to mental retardation and vulnerability to Alzheimer disease in
DS. This mouse demonstrates abnormal behavior and is impaired in various
learning paradigms. We demonstrated that long-term potentiation (LTP) and long-
term depression (LTD) decrease and increase respectively in the CAl region of
the Ts65Dn mouse of Ts65Dn mouse hippocampus. The objective of this proposal is
to find mechanisms that cause the abnormal LTP and LTD. Three specific aims are
proposed to test that abnormal LTP and LTD are due to changes in signal
transduction pathways mediated by protein kinase A (PKA) and/or protein kinase
C (PKC) that would cause posttranslational changes in voltage-dependent Na+,
Ca2+ channels and NMDA-, AMPA-glutamate receptors and cause changes in synaptic
activity in the hippocampus. Aim#1: Determine PKA pathway activity in Ts65Dn
hippocampus in relation to LTP and LTD paradigm. Determine expression of
phosphorylated CREB. Aim #2:PKC activity in Ts65Dn hippocampus in relation to
LTP and LTD paradigm. Determine expression pattern of PKC isoforms. Aim
#3:Determine the expression and posttranslational modification of voltage
dependent Na+, Ca2+ channels and NMDA -, AMPA- glutamate receptors using
nucleated patch-clamp recording technique and binding studies. The impact of
PKC isoforms will be tested in cell lines that permanently over express Na+
channel, Ca2+ channels, NMDA and AMPA receptors. Signal transduction
impairments in genetic model of DS will determine important targets for
treatment of mental retardation. In addition the outcome of this research will
include further understanding of the role of PKA and PKC and voltage-dependent
channels and NMDA- and AMPA-receptors in the mechanisms that are behind LTP and
LTD.
描述(由申请人提供):唐氏综合征(DS)或21三体(Ts 21)是
由21号染色体的三个拷贝的存在引起,并且是最多的
智力迟钝的常见遗传原因。Ts 65 Dn三体小鼠具有
最近被开发,并且是三体的,仅用于鼠的片段
16号染色体与人类21号染色体的片段同源,
导致智力迟钝和易患阿尔茨海默病,
DS.这只小鼠表现出异常行为,
学习范式我们证明了长时程增强(LTP)和长时程增强(LTP)的作用。
在海马CA 1区,长时程抑制(LTD)分别减少和增加,
Ts 65 Dn小鼠海马体的Ts 65 Dn小鼠。本提案的目的是
寻找导致异常LTP和LTD的机制。三个具体目标是
建议测试异常LTP和LTD是由于信号变化引起的
由蛋白激酶A(PKA)和/或蛋白激酶
C(PKC),可引起电压依赖性Na+的翻译后变化,
Ca 2+通道和NMDA-,AMPA-谷氨酸受体,并引起突触的变化,
海马体的活动。目的#1:测定Ts 65 Dn中的PKA途径活性
海马与LTP和LTD范式的关系。确定表达式
磷酸化CREB。目标#2:Ts 65 Dn海马中PKC活性与
LTP和LTD范式。确定PKC亚型的表达模式。目的
#3:确定电压的表达和翻译后修饰
使用依赖性Na+、Ca 2+通道和NMDA -、AMPA-谷氨酸受体
有核膜片钳记录技术和结合研究。的影响
PKC亚型将在永久过度表达Na+的细胞系中进行测试
通道、Ca ~(2+)通道、NMDA和AMPA受体。信号转导
DS遗传模型中的损伤将决定
治疗精神发育迟滞。此外,这项研究的结果将
包括进一步了解PKA和PKC的作用以及电压依赖性
通道和NMDA-和AMPA-受体的机制,是背后的LTP和
公司
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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ZYGMUNT GALDZICKI其他文献
ZYGMUNT GALDZICKI的其他文献
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{{ truncateString('ZYGMUNT GALDZICKI', 18)}}的其他基金
Rescue of Forebrain Defects in Mouse Models of Down syndrome
唐氏综合症小鼠模型前脑缺陷的挽救
- 批准号:
8214387 - 财政年份:2011
- 资助金额:
$ 22.57万 - 项目类别:
Rescue of Forebrain Defects in Mouse Models of Down syndrome
唐氏综合症小鼠模型前脑缺陷的挽救
- 批准号:
8730242 - 财政年份:2011
- 资助金额:
$ 22.57万 - 项目类别:
Rescue of Forebrain Defects in Mouse Models of Down syndrome
唐氏综合症小鼠模型前脑缺陷的挽救
- 批准号:
8531019 - 财政年份:2011
- 资助金额:
$ 22.57万 - 项目类别:
Rescue of Forebrain Defects in Mouse Models of Down syndrome
唐氏综合症小鼠模型前脑缺陷的挽救
- 批准号:
8334486 - 财政年份:2011
- 资助金额:
$ 22.57万 - 项目类别:
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