STUDY & CONTROL OF ANGIOGENESIS AT BIOMATERIAL INTERFACE: SURFACE POROSITY

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• 批准号: 6345060
• 负责人: PAIGE STEPHANS
• 金额: $ 0.74万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6345060
• 关键词: bioengineering /biomedical engineering; biological products; biomaterials; biomedical resource; health care; proteins

We are studying two material factors that may affect angiogenesis. The first, porosity, is based upon previous in vivo work that demonstrated a correlation between porosity and the number of vascular structures located close to the material. The second is coating the material with molecules, such as extracellular or matrix proteins. For example, we are currently immobilizing a peptide from SPARC that has shown angiogenic activity in vivo. To facilitate these studies, we are developing in vitro assays to study angiogenesis in the context of a biomaterial. For example, we have developed an assay to measure migration, a crucial step in angiogenesis. A teflon fence is used to grow endothelial cells to confluence in a well-defined area on the material. Once the fence is removed, the migration on various surfaces can be measured. ESCA is used to characterize the composition and chemistry of the surfaces.

我们正在研究两种可能影响血管生成的物质因素。 第一，孔隙率，是基于以前的体内工作， 证明了孔隙率和血管数量之间的相关性， 靠近材料的结构。 第二个是涂层， 含有分子的材料，例如细胞外蛋白或基质蛋白。 例如，我们目前正在固定化一种来自大肠杆菌的肽， 在体内显示出血管生成活性。 为了促进这些研究， 我们正在开发体外试验来研究血管生成， 一种生物材料。 例如，我们开发了一种检测方法， 迁移是血管生成的关键步骤。 一个铁栅栏是用来 生长内皮细胞，使其在 材料 一旦围栏被拆除， 表面可以测量。 ESCA用于表征 表面的组成和化学性质。