STRUCT FUNCT OF Z DNA BINDING DOMAIN Z OF DSRNA ADENOSINE DEAMINASE TYPE I
DSRNA腺苷脱氨酶I型Z DNA结合域Z的结构功能
基本信息
- 批准号:6355113
- 负责人:
- 金额:$ 2.78万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2000
- 资助国家:美国
- 起止时间:2000-05-01 至 2001-04-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
RNA editing alters pre-mRNA through site-selective adenosine
dearnination, which results in codon changes that lead to the
production of novel proteins. An enzyme that catalyzes this reaction,
double-stranded RNA adenosine deaminase (ADARI), contains two N
terminal Z-DNA-binding motifs, Z,,, and 4, the function of which is as
yet unknown. In this study, multidimensional NMR spectroscopy was
used to show that the topology of Z"' is (X 10 1 (X2 (X3 P2 03.
Long-range NOEs indicate that P1 and P3 interact with each other.
Site-directed mutagenesis was used to identify residues in 0, 03 and
the loop connecting 02 to 03 that affect Z-DNA binding. Also
identified were I I hydrophobic residues that are essential for
protein stability. Comparison with known structures reveals some
similarity between Z,,, and (oc + 0) helix-tum-helix proteins, such as
histone 5 and the family of hepatocyte nuclear factor-3
winged-helix-tum-helix transcription factors. Taken together, the
structural and functional data suggest that recognition of Z-DNA by
Z,,, involves residues in both the 0 helix and the C-terminal P-sheet.
RNA编辑通过位点选择性腺苷改变前mRNA
脱氨,这导致密码子的变化,导致
生产新的蛋白质。 催化这种反应的酶,
双链RNA腺苷脱氨酶(ADARI),含有两个N
末端Z-DNA结合基序,Z,,和4,其功能如
还不知道 在这项研究中,多维核磁共振光谱,
用于表明Z”'的拓扑是(X 10 1(X2(X3 P2 03.
长程NOE表明P1和P3相互作用。
使用定点诱变来鉴定0、03和04中的残基。
连接02和03的环影响Z-DNA结合。 也
鉴定出的11个疏水残基是必需的,
蛋白质稳定性 与已知结构的比较揭示了一些
Z、、和(α + 0)螺旋-转角-螺旋蛋白之间的相似性,例如
组蛋白5与肝细胞核因子3家族
翼-螺旋-反转-螺旋转录因子。 总的来说,
结构和功能数据表明,Z-DNA识别
Z,,涉及0螺旋和C-末端P-折叠中的残基。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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STRUCT FUNCT OF Z DNA BINDING DOMAIN Z OF DSRNA ADENOSINE DEAMINASE TYPE I
DSRNA腺苷脱氨酶I型Z DNA结合域Z的结构功能
- 批准号:
6118680 - 财政年份:1999
- 资助金额:
$ 2.78万 - 项目类别: