ANTIOXIDANT POTENTIAL OF HOMOCYSTAMIDE LDL ADDUCT
同型半酰胺低密度脂蛋白加合物的抗氧化潜力
基本信息
- 批准号:6307877
- 负责人:
- 金额:$ 1.13万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2000
- 资助国家:美国
- 起止时间:2000-03-01 至 2001-02-28
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Homocysteine thiolactone is a cyclic thioester that is implicated
in atherogenesis. This molecule will readily acylate primary amines,
forming a homocystamide adduct, which contains a primary amine and a
thiol. Here, we have characterized and evaluated the antioxidant
potential of the homocystamide-low-density lipoprotein (LDL) adduct, a
product of the acylation reaction between homocysteine thiolactone and
LDL. Treatment of LDL with homocysteine thiolactone resulted in a
time-dependent increase in LDL-bound thiol content that reached
approximately 250 nmol thiol/mg LDL protein at 75 minutes. The
increase in LDL thiol content was followed by aggregation of LDL after
75 minutes. The increase in LDL-bound thiols was reversible by
treatment with the thiol blockersing spin label, methanethiosulfonate.
As assessed by the electron spin resonance (ESR) spin labeling
technique, the homocystamide adducts were predominately exposed to the
aqueous phase of LDL, shown by the sharp ESR spectra that were greatly
broadened by the hydrophillic paramagnetic relaxing agent, chromium
oxalate. The relative electrophoretic mobility of the
homocystamide-LDL adduct was increased with respect to native LDL.
Primary amino group concentration of homocysteine thiolactone-treated
LDL was not significantly different than native LDL (p < 0.05). The
homocystamide-LDL adduct was resistant to Cu(2+)- and 2,2'-azobis
(2-amidinopropane)- mediated oxidation (with respect to native LDL) as
measured by the formation of thiobarbituric reactive substances and
the depletion of vitamin E. Blocking thiols with N-ethylmaleamide
prevented the antioxidant effect of the homocystamide-LDL adduct. The
potential relationship between the homocystamide-LDL adduct and the
development of atherosclerosis is discussed.
同型半胱氨酸硫内酯是一种环状硫酯
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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ERIC FERGUSON其他文献
ERIC FERGUSON的其他文献
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{{ truncateString('ERIC FERGUSON', 18)}}的其他基金
MECHANISM OF APOLIPOPROTEIN B 100 THIOL DEPLETION DURING OXIDATION OF LDL
LDL 氧化过程中载脂蛋白 B 100 硫醇消耗的机制
- 批准号:
6307907 - 财政年份:2000
- 资助金额:
$ 1.13万 - 项目类别:
POLYCLONARL ANTIBODY DIRECTED AGAINST HOMOCYSTEINE THIOLACTONE MODIFIEDLDL
针对高半胱氨酸硫内酯修饰LDL的多克隆抗体
- 批准号:
6307906 - 财政年份:2000
- 资助金额:
$ 1.13万 - 项目类别:
POLYCLONAL ANTIBODY DIRECTED AGAINST HOMOCYSTEINE THIOLACTONE MODIFIED LDL
针对同型半胱氨酸硫内酯修饰的 LDL 的多克隆抗体
- 批准号:
6118862 - 财政年份:1999
- 资助金额:
$ 1.13万 - 项目类别:
POLYCLONARL ANTIBODY DIRECTED AGAINST HOMOCYSTEINE THIOLACTONE MODIFIEDLDL
针对高半胱氨酸硫内酯修饰LDL的多克隆抗体
- 批准号:
6279881 - 财政年份:1998
- 资助金额:
$ 1.13万 - 项目类别:
MECHANISM OF APOLIPOPROTEIN B 100 THIOL DEPLETION DURING OXIDATION OF LDL
LDL 氧化过程中载脂蛋白 B 100 硫醇消耗的机制
- 批准号:
6279882 - 财政年份:1998
- 资助金额:
$ 1.13万 - 项目类别:
ANTIOXIDANT POTENTIAL OF HOMOCYSTAMIDE LDL ADDUCT
同型半酰胺低密度脂蛋白加合物的抗氧化潜力
- 批准号:
6279846 - 财政年份:1998
- 资助金额:
$ 1.13万 - 项目类别:
RADICAL MEDIATED APOLIPOPROTEIN B 100 THIOL DEPLETION
自由基介导的载脂蛋白 B 100 硫醇消耗
- 批准号:
6250044 - 财政年份:1997
- 资助金额:
$ 1.13万 - 项目类别:
HOMOCYSTEINE IN MODIFICATION OF LOW DENSITY LIPOPROTEIN
同型半胱氨酸修饰低密度脂蛋白
- 批准号:
6250043 - 财政年份:1997
- 资助金额:
$ 1.13万 - 项目类别:
APOLIPOPROTEIN B 100 THIOL DEPLETION DURING OXIDATION OF LDL
LDL 氧化过程中载脂蛋白 B 100 硫醇消耗
- 批准号:
5222143 - 财政年份:
- 资助金额:
$ 1.13万 - 项目类别:
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