ZEISS LSM 510 CONFOCAL MICROSCOPE

在过去的三年里，范德比尔特的数字成像技术的使用迅速增长。 其中一些是由于新的教师，但其中大部分是由于成功的细胞成像共享资源在教学研究人员使用共聚焦显微镜不能满足一个仪器，目前可供范德比尔特教师。 为了弥补这一缺陷，现有共聚焦显微镜的几个成熟用户开始讨论一项计划，用于分担新仪器的使用和维护成本。 主要用户包括在这个建议妥协的核心组共享相关的研究目标，并采用类似的技术。 例如，Denison和Solnica-Krezel都在对活细胞和胚胎进行延时成像实验，至少还有另外两名研究人员（Hogan，Wright）预计将使用这种方法。 PI，米勒，与戴夫活塞，细胞成像资源的主任合作，已经开发了在LSM 510多色GFP成像的方法。 Denison、Solnica-Kerezel和米勒打算在他们的项目中利用GFP多路复用。 主要用户的Severak将使用免疫化学或细胞学染料（Denison、Hogan、Wright、Solnica-Kerezel）在固定组织中进行荧光标记。 LSM 510（458、488、514、633）的计算机控制的多条激光线提供了这些研究人员所需的全范围激发波长和功率。 虽然显微镜将主要由主要用户使用，但显微镜时间将通过细胞成像共享资源分配，以确保分配公平，并且计划外的时间可由范德比尔特的其他研究者使用。

There has been a rapid growth in the use of digital imaging at Vanderbilt over the last three years. Some of this due to newly faculty, but much of it has arisen from success of the Cell Imaging Shared Resource in teaching researchers to use the confocal microscopy cannot be met by the one instrument that is currently available to Vanderbilt faculty. In an effort to remedy this deficit, several well established users of the existing confocal microscope began discussion a plan for sharing the use and maintenance costs of a new instrument. The Major Users included in this proposal compromise a core group share related research goals and employ similar techniques. For example, both Denison and Solnica-Krezel are performing time-lapse imaging experiments on live cells and embryos and at least two other investigators (Hogan, Wright) anticipate using this approach. The PI, Miller, in collaboration with Dave Piston, the Director of the Cell Imaging Resource, have developed methods for multicolor-GFP imaging in the LSM510. Denison, Solnica-Kerezel and Miller intend to exploit GFP multiplexing in their projects. Severak of the Major Users will employ multicolor labeling in fixed tissues with immunochemical or cytological dyes (Denison, Hogan, Wright, Solnica-Kerezel). The computer controlled, multiple laser lines of the LSM510 (458, 488, 514, 633) provide the full range of excitation wavelengths and powers needed by these investigators. Although the microscope will be largely utilized by the Major Users, microscope time will be allocated through the Cell Imaging Shared Resource to insure that allotments are fair and that unscheduled time can be used by other investigators at Vanderbilt.