LASER CAPTURE MICROSCOPE

激光捕获显微镜

• 批准号: 6288079
• 负责人: CAROL K PETITO
• 金额: $ 12.31万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2002-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6288079
• 关键词: biomedical equipment purchase; microscopy

The University of Miami School of Medicine requests a laser capture microscope (LCM) for its NIH-funded investigators. This state-of-the-art instrument is automated and user-friendly equipment that provides for the microdissected tissue of individual cells or groups of cells from tissue sections. DNA, RNA and protein can be isolated from the microdissected tissue and is of sufficient quality and quantity for use for gene amplification by polymerase chain reaction (PCR) or reverse transcriptase (RT)-PCR, clonal analysis and direct sequencing, microchip arrays for differential display; and Western Blot and 2-dimensional protein electrophoresis. Microdissection provides a means to evaluate only the specific tissue of interest and avoids contamination by neighboring cells and tissues and by intravascular blood products. Unquestionably, LCM technology assists scientists in any field of medical and biological science and it is likely to be a standard, state of the art piece of equipment in any medical school, analogous to the electron microscope when that instrument was first introduced commercially available. An LCM instrument is not available locally and those of us who have experience with the LCM have been required to travel to distant academic centers to do so. Investigators at the University of Miami require such a facility to enrich and enhance our current NIH-funded research projects and enable us to conduct our research endeavors with cutting-edge technology. Institutional support for this LCM facility is enthusiastic and strong. The Specific Aims of this Shared Instrumentation Grant are to 1) set up and operate a fully equipped central core LCM laboratory; 2) provide a structured environment for the evaluation of proposed, on-going and completed research projects utilizing the LCM laboratory; 3) train investigators at the University of Miami and other south Florida are not of sufficient quality for publication prints and for presentations at national and international meetings. Consequently, we request a camera and a workstation for the double headed microscope. This will be exclusively be utilized by Users of the LCM facility.

迈阿密大学医学院为其NIH资助的研究人员申请激光捕获显微镜（LCM）。 这种最先进的仪器是自动化和用户友好的设备，可从组织切片中显微解剖单个细胞或细胞组的组织。 DNA、RNA和蛋白质可以从显微切割的组织中分离出来，并且具有足够的质量和数量，用于通过聚合酶链反应（PCR）或逆转录酶（RT）-PCR进行基因扩增、克隆分析和直接测序、用于差异显示的微芯片阵列;以及蛋白质印迹和二维蛋白质电泳。 显微切割提供了一种仅评估特定目标组织的方法，避免了邻近细胞和组织以及血管内血液制品的污染。 毫无疑问，LCM技术可以帮助医学和生物科学任何领域的科学家，它很可能是任何医学院的标准设备，类似于电子显微镜，当该仪器首次推出商业化时。 当地没有LCM仪器，我们这些有LCM经验的人必须前往遥远的学术中心。 迈阿密大学的研究人员需要这样一个设施来丰富和加强我们目前的NIH资助的研究项目，使我们能够利用尖端技术进行我们的研究工作。 对这一LCM机制的机构支持热情而有力。该共享仪器补助金的具体目标是：1）建立和运营一个设备齐全的中央核心LCM实验室; 2）提供一个结构化的环境，用于评估利用LCM实验室进行的拟议，正在进行和已完成的研究项目;第三章迈阿密大学和其他南佛罗里达大学的火车调查员的质量不足以出版印刷品和在国家和国际会议上的演讲，国际会议。 因此，我们要求为双头显微镜配备一台相机和一个工作站。 这将专门由LCM设施的用户使用。