MULTIPHOTON SCANNING LASER FLUORESCENCE MICROSCOPE

多光子扫描激光荧光显微镜

• 批准号: 6291330
• 负责人: WILLIAM M ROBERTS
• 金额: $ 50万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291330
• 关键词: biomedical equipment purchase; fluorescence microscopy

This Shared Instrumentation Grant proposal requests funds to purchase a Zeiss LSM 510 NLO multiphoton laser scanning microscope, to be installed in the Bio-Optics Laboratory at the University of Oregon. This microscope will be used to advance ongoing NIH supported research in numerous laboratories in the Institutes of Neuroscience and Molecular Biology at the University of Oregon, including six major users who have described specific projects that will be greatly facilitated by the availability of a multi-photon fluorescence microscope. The two main benefits offered by this new technology are (1) the capability performing time-lapse microscopy on living specimens with greatly reduced photodynamic damage or bleaching of fluorescent labels and (2) the ability to obtain crisp images at depths of 100 mu m or more into a specimen. Five of the major users study the developmental biology of zebrafish (Judith Eisen, Charles Kimmel and John Postlethwait), Drosophila (Chris Doe), tunicates (John Postlethwait) and C. elegans (Bruce Bowerman). One user (William Roberts) studies synaptic physiology and calcium signaling in sensory receptors in the frog ear. The microscope will be available as a shared facility for use by life scientists at the University of Oregon.

该共享仪器拨款提案要求提供资金购买蔡司 LSM 510 NLO 多光子激光扫描显微镜，并将其安装在俄勒冈大学生物光学实验室。该显微镜将用于推进俄勒冈大学神经科学和分子生物学研究所众多实验室正在进行的 NIH 支持的研究，其中包括六位主要用户，他们描述了多光子荧光显微镜的可用性将极大促进的具体项目。这项新技术提供的两个主要优点是（1）能够对活体标本进行延时显微镜检查，大大减少光动力损伤或荧光标记的漂白；（2）能够在标本内部 100 微米或更深的深度获得清晰的图像。其中五个主要用户研究斑马鱼（Judith Eisen、Charles Kimmel 和 John Postlethwait）、果蝇（Chris Doe）、被囊动物（John Postlethwait）和秀丽隐杆线虫（Bruce Bowerman）的发育生物学。一位用户（威廉·罗伯茨）研究青蛙耳朵感觉受体的突触生理学和钙信号传导。该显微镜将作为共享设施供俄勒冈大学的生命科学家使用。