MULTIPHOTON SCANNING LASER FLUORESCENCE MICROSCOPE

这份共享仪器助学金计划申请资金购买蔡司LSM 510NLO多光子激光扫描显微镜，安装在俄勒冈大学的生物光学实验室。这种显微镜将被用于推进俄勒冈大学神经科学和分子生物学研究所众多实验室正在进行的NIH支持的研究，其中包括六个主要用户，他们描述了一些具体的项目，多光子荧光显微镜的问世将极大地促进这些项目的开展。这项新技术提供的两个主要好处是(1)能够在活标本上进行延时显微镜检查，大大减少了荧光标签的光动力损伤或漂白；(2)能够在100微米或更深的深度获得清晰的图像。五个主要使用者研究斑马鱼(朱迪思·艾森、查尔斯·基梅尔和约翰·波斯尔斯韦特)、果蝇(克里斯·多伊)、被囊动物(约翰·波斯尔斯韦特)和线虫(布鲁斯·鲍尔曼)的发育生物学。一位使用者(威廉·罗伯茨)研究了青蛙耳朵感觉受体中的突触生理学和钙信号。该显微镜将作为共享设备提供给俄勒冈大学的生命科学家使用。

This Shared Instrumentation Grant proposal requests funds to purchase a Zeiss LSM 510 NLO multiphoton laser scanning microscope, to be installed in the Bio-Optics Laboratory at the University of Oregon. This microscope will be used to advance ongoing NIH supported research in numerous laboratories in the Institutes of Neuroscience and Molecular Biology at the University of Oregon, including six major users who have described specific projects that will be greatly facilitated by the availability of a multi-photon fluorescence microscope. The two main benefits offered by this new technology are (1) the capability performing time-lapse microscopy on living specimens with greatly reduced photodynamic damage or bleaching of fluorescent labels and (2) the ability to obtain crisp images at depths of 100 mu m or more into a specimen. Five of the major users study the developmental biology of zebrafish (Judith Eisen, Charles Kimmel and John Postlethwait), Drosophila (Chris Doe), tunicates (John Postlethwait) and C. elegans (Bruce Bowerman). One user (William Roberts) studies synaptic physiology and calcium signaling in sensory receptors in the frog ear. The microscope will be available as a shared facility for use by life scientists at the University of Oregon.