MSC BLUE-3 AND MSC PURPLE-3 CONFOCAL X-RAY OPTICS

MSC BLUE-3 和 MSC Purple-3 共焦 X 射线光学器件

• 批准号: 6292221
• 负责人: Adrian R. Ferre-D'Amare
• 金额: $ 11.95万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6292221
• 关键词: X ray crystallography; bioimaging /biomedical imaging; biomedical equipment purchase; macromolecule; optics; structural biology

This shared instrumentation proposal from the structural biology program at the Fred Hutchinson Cancer Research Center requests funding for the acquisition of high-performance, graded multilayer X-ray optics. These will update and maximize the capabilities of an existing macromolecular crystallographic data-collection system employed by five principal investigators and their laboratories. The upgrade of this integrated instrument system will greatly facilitate ongoing collaborative efforts both within the center and with a large number of molecular biology and biochemistry laboratories throughout the country. The macromolecular structure program at the Hutchinson Center was established approximately seven years ago as a response to a clear need among the molecular biology laboratories at the Center for collaborative structure/function studies which support their research programs in genetic regulation, developmental biology, signal transduction, cell cycle control, and other basic biological phenomena. In addition, all five principal investigators listed below actively conduct their own original research in such areas as structural enzymology, time-resolved crystallography, viral protein structure, protein-nucleic acid recognition, RNA structure, immune system recognition and response, protein engineering, crystallographic phasing, and protein folding. It is thus necessary to maximize the capabilities of our X-ray equipment to satisfy our requirements for efficient and accurate data collection. The goal of this proposal is to update cost-effectively our shared diffraction facility by replacing suboptimal graphite monochromator and Franks-type mirrors with state-of-the-art graded multilayer X-ray optics. We are requesting funds to purchase MSC/Max-Flux confocal optics that will result in up to 27-fold increase in useable X-ray flux, relative to the current configuration.

弗雷德哈钦森癌症研究中心的结构生物学项目的这一共享仪器提案要求为收购高性能、分级多层X射线光学器件提供资金。这将更新和最大限度地提高现有的大分子晶体学数据收集系统的能力，该系统由五名主要研究人员及其实验室使用。该集成仪器系统的升级将极大地促进中心内部以及与全国大量分子生物学和生物化学实验室的持续合作。哈钦森中心的大分子结构计划是在大约七年前建立的，作为对该中心分子生物学实验室之间合作结构/功能研究的明确需求的回应，这些研究支持他们在遗传调控、发育生物学、信号转导、细胞周期控制和其他基本生物现象方面的研究计划。此外，下面列出的所有五个主要研究人员积极进行自己的原创性研究，如结构酶学，时间分辨晶体学，病毒蛋白质结构，蛋白质-核酸识别，RNA结构，免疫系统识别和反应，蛋白质工程，晶体定相和蛋白质折叠。因此，有必要最大限度地提高我们的X射线设备的能力，以满足我们对有效和准确的数据收集的要求。该提案的目标是通过用最先进的渐变多层X射线光学器件取代次优的石墨单色器和弗兰克型反射镜，以经济有效地更新我们的共享衍射设备。我们正在申请资金购买MSC/Max-Flux共聚焦光学器件，这将导致可用X射线通量相对于当前配置增加27倍。

项目成果

The roles of metal ions in regulation by riboswitches.

• DOI: 10.1039/9781849732512-00141
• 发表时间: 2011
• 期刊: Metal ions in life sciences
• 作者: Ferré-D'Amaré AR;Winkler WC
• 通讯作者: Winkler WC

U1A RNA-binding domain at 1.8 A resolution.

U1A RNA 结合域，分辨率为 1.8 A。

• DOI: 10.1107/s0907444903011338
• 发表时间: 2003
• 期刊: Acta crystallographica. Section D, Biological crystallography
• 作者: Rupert,PeterB;Xiao,Hong;Ferré-D'Amaré,AdrianR
• 通讯作者: Ferré-D'Amaré,AdrianR

Structural basis for specific, high-affinity tetracycline binding by an in vitro evolved aptamer and artificial riboswitch.

• DOI: 10.1016/j.chembiol.2008.09.004
• 发表时间: 2008-10-20
• 期刊: Chemistry & biology
• 作者: Xiao H;Edwards TE;Ferré-D'Amaré AR
• 通讯作者: Ferré-D'Amaré AR

The glmS ribozyme: use of a small molecule coenzyme by a gene-regulatory RNA.

• DOI: 10.1017/s0033583510000144
• 发表时间: 2010-11
• 期刊: QUARTERLY REVIEWS OF BIOPHYSICS
• 影响因子: 6.1
• 作者: Ferre-D'Amare, Adrian R.

The hairpin ribozyme: from crystal structure to function.

发夹核酶：从晶体结构到功能。

• DOI: 10.1042/bst0301105
• 发表时间: 2002
• 期刊: Biochemical Society transactions
• 影响因子: 3.9
• 作者: Ferré-D'amaré,AR;Rupert,PB
• 通讯作者: Rupert,PB