ACQUISITION OF AN 800 MHZ NMR SPECTROMETER

我们建议收购一台800 MHz NMR光谱仪，主要为UCSF的研究社区服务，并为加州北方其他大学加州校区的研究人员提供服务。为新仪器提出的研究将提高我们使用多维NMR实验确定溶液中主要是蛋白质，核酸以及蛋白质和核酸复合物的高分辨率结构的能力，作为阐明生物功能的一种手段。新仪器提供的额外灵敏度和分辨率，以及对残余偶极耦合测量和结合横向弛豫优化光谱（TROSY）的脉冲序列的增强利用，不仅将增加结构测定的吞吐量，而且将允许在目前模糊的区域中测定共振分配，能够确定导致更高质量结构的附加结构约束，能够确定由于聚集问题而至今不可能的一些结构，并扩展我们确定分子量>30千道尔顿的生物聚合物结构的能力。

We propose the acquisition of an 800 MHz NMR spectrometer that will serve the research community of UCSF primarily with some service for researchers on other University of California campuses in Northern California. The research proposed for the new instrument will improve our capability to use multidimensional NMR experiments for determining high-resolution structures in solution primarily of proteins, nucleic acids, and complexes entailing proteins and nucleic acids, as a means of elucidating biological function. The additional sensitivity and resolution, as well as enhanced utilization of residual dipolar coupling measurements and pulse sequences incorporating transverse relaxation optimized spectroscopy (TROSY), afforded by the new instrument will not only increase through-put in structure determination, but it will permit determination of resonance assignments in regions which are currently ambiguous, enable additional structural restraints to be determined leading to higher quality structures, enable some structure determinations which have not been possible so far due to aggregation problems, and extend our ability to determine structures of biopolymers with molecular mass >30 kilodaltons.