T CELL ACTIVATION IN HIV 1 REVERSE TRANSCRIPTION/INTEGRA

描述：(改编自调查人员摘要)在本提案中， 一位年轻的研究人员希望研究蛋白质是否参与T细胞激活 可能用于HIV-1的逆转录。为她提供便利 研究表明，她已经获得了初步数据，证明T细胞 蛋白质与生物素标记的HIV-1单链DNA和RNA结构相结合 使用电泳迁移率变化的非放射性适配 化验。使用这项技术，调查员建议调查 HIV-1逆转录中间体与T细胞的相互作用 蛋白质来检验早期和有效逆转的假说 HIV-1单链DNA中间产物转录可能有助于HIV-1感染 通过为参与其中的宿主细胞蛋白提供结合位点而进行的过程 整合前或整合事件。为了这个目标，调查员 提出以下具体目标：具体目标1：划定 HIV-1RNA、单链DNA、RNA DNA上的特异位点杂交体构建 与T细胞蛋白相互作用。具体目标2：调查 HIV-1逆转录中间产物与HIV-1的相互作用 整合酶蛋白

DESCRIPTION: (Adapted from investigator's abstract) In this proposal, a young investigator wishes to study if proteins involved in T cell activation might be used in the HIV-1 reverse transcription. To facilitate her studies, she has obtained preliminary data demonstrating that T cell proteins bind to biotinylated HIV-1 single stranded DNA and RNA constructs using a non-radioactive adaptation of the electrophoretic mobility shift assay. Using this technique, the investigator proposes to investigate the interaction of HIV-1 reverse transcription intermediates with T cell proteins to test the hypothesis that early and efficient reverse transcription of HIV-1 ssDNA intermediates may assist HIV-1 in the infection process by providing a binding site for host cell proteins involved in pre-integration or integration events. Towards this goal, the investigator proposes the following Specific Aims: Specific Aim 1: To delineate the specific sites on HIV-1 RNA, single stranded DNA, RNA DNA hybrid constructs interacting with T cell proteins. Specific Aim 2: To investigate interactions between HIV-1 reverse transcription intermediates and the HIV-1 integrase protein