Core--Transgenic and knockout mouse

核心--转基因及基因敲除小鼠

• 批准号: 6325965
• 负责人: ANNEMARIE WALSH
• 金额: $ 26.82万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6325965
• 关键词: animal colony; atherosclerosis; biomedical facility; blood lipoprotein metabolism; cryopreservation; gene targeting; genetically modified animals; transfection

The transgenic and knockout mouse core will support the work of all 4 projects of the proposed PPG. The core will be located at the Rockefeller University Laboratory Animal Research Center. The core will: 1) micro- inject DNA into fertilized eggs to create transgenic mouse lines; 2) electroporate DNA constructions into ES cells, clone and select homologous recombinants, perform blastocyst injection of correctly targeted ES cells, breed chimeras to demonstrate germ line transmission to demonstrate germ line transmission of gene knockout; and 3) cryopreserve liens of transgenic and knockout mice.

转基因和淘汰小鼠核心将支持拟议的PPG的所有4个项目的工作。核心将位于洛克菲勒大学实验室动物研究中心。核心将：1）将DNA微注入受精卵以产生转基因小鼠系； 2）电塑料DNA构建体到ES细胞，克隆并选择同源重组剂，进行胚泡注射正确靶向的ES细胞，繁殖嵌合体以证明生殖系传播以证明基因敲除的生殖线传递； 3）转基因和敲除小鼠的冷冻保存。