HUMAN CELL CULTURE STUDIES OF MUTAGENS IN THE ABERJONA BASIN

阿伯霍纳盆地诱变剂的人类细胞培养研究

• 批准号: 6340888
• 负责人: William G Thilly
• 金额: $ 17.53万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6340888
• 关键词: B lymphocyte; arsenic; biomarker; chromium; denaturing gradient gel electrophoresis; environmental toxicology; family genetics; gel electrophoresis; gene mutation; hazardous substances; human genetic material tag; human population genetics; human subject; human tissue; industrial waste; method development; mitochondrial DNA; mutagens; nucleic acid sequence; polymerase chain reaction; ribosomal RNA; tissue /cell culture; toxicant interaction; waste disposal; water pollution

Description: River and lake sediments from the Aberjona Valley have been shown to mutate human cells in short term assays. The chemical responsible for most of the mutagenicity of the organic sediment phase has been identified as benzo(a)pyrene. Its abundance correlates well with the local use of coal for home heating over the past century. The inorganic chemical mutagenicity of sediments of the Aberjona is however much greater than the organic group. Most of this activity may be ascribed to chromate deposited after two major releases circa 1930 and 1960 from the Aberjona headwater in and about the present Industriplex Superfund Site. Humans are usually exposed to water-borne chemicals at concentrations low enough to justify an assumption of independent action. This is claimed not to be the case for our estimate for chromate (4muM) and arsenite (1muM) in East Woburn drinking water drawn from municipal wells G and H. Thus, the intention is to examine the mutagenic results of arsenite interaction with chromate and other mutagens. Furthermore, human body water concentrations would be significantly lower than drinking water levels. They therefore consider it necessary to examine the mutagenicity of the sediments' chemicals at concentrations covering the range expected for children from birth to adolescence. In addition, they propose to extend these studies of stream and lake sediments into the two additional sub-basins which feed the Mystic Lakes proposed for study in the hydrogeology projects 1-6. The proposal includes obtaining mutational spectra from human cell cultures exposed to raw river sediments, chromate and chromate/arsenite mixtures. Long-term low dose protocols will be used to recreate the conditions calculated to have existed in children drinking the contaminated water from wells G and H. This project combined with the core laboratory and project 1 is responsible for identifying human mutagens in the Aberjona basin. Short term tests are carried out on sediments and the mutagens identified. As chromate and arsenite have been identified in well water, the influence of these and other mutagenic compounds are to be tested in human cell cultures. The wells providing drinking water for Woburn MA residents contain 4uM chromate and 1 uM in arsenite in the period 1964 - 1979. The specific aims of this project is to determine the biological meaning of exposure to such chemicals.

描述：来自阿伯乔纳山谷的河流和湖泊沉积物已经被 在短期试验中显示出突变人类细胞。化学 负责有机沉积物相的大部分致突变性 已被鉴定为苯并（a）芘。它的丰度与 随着过去一个世纪当地使用煤炭进行家庭取暖。的 然而，Aberjona沉积物的无机化学致突变性 远远大于有机基团。大部分活动可能是 归因于大约1930年两次主要释放后沉积的铬酸盐， 1960年，从阿伯乔纳源头进入现在的工业园区 超级基金网站。 人类通常暴露于浓度较低的水传播化学物质 足以证明独立行动的假设。这是声称 而我们对铬酸盐（4 μ M）和亚砷酸盐的估计则不是这样 （1 μ M）在东沃本饮用水从市政威尔斯G井和 H.因此，目的是检查亚砷酸盐的致突变结果 与铬酸盐和其他诱变剂相互作用。此外，人体 水的浓度将大大低于饮用水 程度.因此，他们认为有必要研究 沉积物化学品的致突变性，其浓度涵盖 从出生到青春期的预期范围。此外他们 建议将这些河流和湖泊沉积物的研究扩展到 另外两个为神秘湖提供水源的子盆地被提议进行研究 水文地质项目1-6。 该提案包括从人类细胞中获得突变谱 暴露于原始河流沉积物、铬酸盐和铬酸盐/亚砷酸盐的培养物 混合物。长期低剂量方案将用于重建 计算出的儿童饮用 来自威尔斯G和H井的污染水。 本项目结合核心实验室和项目1， 负责在阿伯乔纳盆地鉴定人类诱变剂短 对沉积物进行了长期试验，并确定了诱变剂。 由于在井水中发现了铬酸盐和亚砷酸盐， 这些和其它致突变化合物影响将在 人类细胞培养物。为马萨诸塞州沃本提供饮用水的威尔斯井 1964 - 2000年，居民体内含有4 μ M铬酸盐和1 μ M亚砷酸盐， 1979.该项目的具体目标是确定生物 暴露于这些化学品的含义。