HUMAN CELL CULTURE STUDIES OF MUTAGENS IN THE ABERJONA BASIN

描述：阿伯乔纳河谷的河流和湖泊沉积物已经 在短期测试中被证明能使人类细胞发生突变。化学物质 对有机沉淀相的大部分致突变性负责 经鉴定为苯并(A)芘。其丰度很好地与 在过去的一个世纪里，当地家庭取暖使用了煤炭。这个 然而，阿伯乔纳河沉积物的无机化学致突变性 比有机基团大得多。这些活动中的大部分可能是 可归因于大约1930年和1930年左右两次主要释放后沉积的铬酸盐 1960年从阿伯乔纳源头出发，在现在的工业园区内和周围 超级基金网站。 人类通常暴露在低浓度的水基化学品中。 足以证明采取独立行动的假设是合理的。这是声称的 我们对铬酸盐(4微米)和亚砷酸盐的估计不是这样的 (1微米)东沃本饮用水取自市政G井和G井 H.因此，目的是检查亚砷酸盐的诱变结果 与铬酸盐和其他诱变剂的相互作用。此外，人体 水的浓度将大大低于饮用水 级别。因此，他们认为有必要研究 沉积物中化学物质的致突变性 儿童从出生到青春期的预期范围。此外，他们还 建议将这些对河流和湖泊沉积物的研究扩展到 建议研究的另外两个补充神秘湖泊的子盆地 在水文地质项目1-6中。 该提议包括从人类细胞中获得突变谱 接触未经处理的河流沉积物、铬酸盐和铬酸盐/亚砷酸盐的培养 混合物。长期的低剂量方案将被用来重建 据计算，饮用这种药物的儿童存在的情况 G井和H井的受污染的水。 该项目与核心实验室和项目1相结合，是 负责在阿伯乔纳盆地识别人类诱变物。短的 对沉积物进行术语测试，并确定诱变剂。 由于井水中已发现铬酸盐和亚砷酸盐， 这些化合物和其他诱变化合物的影响将在 人类细胞培养。为马萨诸塞州沃本提供饮用水的水井 居民在1964年期间含有4微米铬酸盐和1微米亚砷酸盐- 1979年。这个项目的具体目标是确定生物 接触这类化学物质的意义。

Description: River and lake sediments from the Aberjona Valley have been shown to mutate human cells in short term assays. The chemical responsible for most of the mutagenicity of the organic sediment phase has been identified as benzo(a)pyrene. Its abundance correlates well with the local use of coal for home heating over the past century. The inorganic chemical mutagenicity of sediments of the Aberjona is however much greater than the organic group. Most of this activity may be ascribed to chromate deposited after two major releases circa 1930 and 1960 from the Aberjona headwater in and about the present Industriplex Superfund Site. Humans are usually exposed to water-borne chemicals at concentrations low enough to justify an assumption of independent action. This is claimed not to be the case for our estimate for chromate (4muM) and arsenite (1muM) in East Woburn drinking water drawn from municipal wells G and H. Thus, the intention is to examine the mutagenic results of arsenite interaction with chromate and other mutagens. Furthermore, human body water concentrations would be significantly lower than drinking water levels. They therefore consider it necessary to examine the mutagenicity of the sediments' chemicals at concentrations covering the range expected for children from birth to adolescence. In addition, they propose to extend these studies of stream and lake sediments into the two additional sub-basins which feed the Mystic Lakes proposed for study in the hydrogeology projects 1-6. The proposal includes obtaining mutational spectra from human cell cultures exposed to raw river sediments, chromate and chromate/arsenite mixtures. Long-term low dose protocols will be used to recreate the conditions calculated to have existed in children drinking the contaminated water from wells G and H. This project combined with the core laboratory and project 1 is responsible for identifying human mutagens in the Aberjona basin. Short term tests are carried out on sediments and the mutagens identified. As chromate and arsenite have been identified in well water, the influence of these and other mutagenic compounds are to be tested in human cell cultures. The wells providing drinking water for Woburn MA residents contain 4uM chromate and 1 uM in arsenite in the period 1964 - 1979. The specific aims of this project is to determine the biological meaning of exposure to such chemicals.