IL 1 INDUCED EXPRESSION OF MONOCYTE CHEMOATTRACTANT PROTEIN W/ TYPE I DIABETES
IL 1 诱导 I 型糖尿病单核细胞趋化蛋白的表达
基本信息
- 批准号:6336799
- 负责人:
- 金额:$ 0.6万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2000
- 资助国家:美国
- 起止时间:2000-08-01 至 2001-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Insulitis, inflammation due to infiltration of immune cells in and
around pancreatic islets, is a hallmark of type I diabetes. Different
stages of insulitis have been observed in non-obese diabetic (NOD)
mice and BioBreeding (BB) rats. Mechanisms involved in the
destructive progression of insulitis, however, are not clearly
understood. In this study, we examined the role of the cytokine
interluekin-1 (IL-1) in stimulating islet cells to produce a
chemotactic factor(s) that may play a role in the progression of
insulitis. IL-1 induces accumulation of non-=esterified arachidonic
acid in islets as demonstrated by isotope dilution mass spectrometry.
Some arachidonate metabolites have chemotactic activity. We have
observed that conditioned media collected from rat islets treated with
5 units/ml IL-1 contained chemotactic activities for human monocytes
determined by modified Boyden chamber assays. The production of
chemotactic activity by islets exposed to IL-1 was
time-dep endent and completely blocked by treating islets with 1(M
actinomycin D. Addition of antiserum raised against rat macrophage
chemoattractant protein-1 (MCP-1) to conditioned media significantly
inhibited monocyte chemotaxis, suggesting that MCP-1 may, in part, be
responsible for the chemotactic activity observed. We confirmed the
synthesis of MCP-1 mRNA by rat islets using in situ hybridization.
(-cells of the istlet appear to be the source of MCP-1 since primary
(-cells and the insulinoma cell line, RINm5F, express MCP-1 mRNA upon
IL-1 activation. These results indicate that pancreatic (-cells
produce MCP-1 upon IL-1 activation that may play a role in the
progression of insulitis associated with autoimmune diabetes.
岛炎,因免疫细胞渗入而引起的炎症
在胰岛周围,是I型糖尿病的标志。不同
在非肥胖型糖尿病(NOD)中观察到了不同阶段的胰岛素炎。
小鼠和生物育种(BB)大鼠。参与其中的机制
然而,胰岛素炎的破坏性进展尚不清楚。
明白了。在这项研究中,我们研究了细胞因子的作用。
白介素1(IL-1)在刺激胰岛细胞产生
趋化因子(S),可能在肺癌的进展中发挥作用
胰岛炎症。IL-1诱导非酯化花生四烯酸蓄积
用同位素稀释质谱仪证实了胰岛中的酸。
一些花生四烯酸代谢产物具有趋化活性。我们有
观察到从大鼠胰岛收集的条件培养液
5U/mlIL-1对人单核细胞的趋化活性
用改良的博伊登小室测定法测定。产品的生产
IL-1对胰岛趋化活性的影响
用1(M)处理胰岛可延缓和完全阻断胰岛
放线菌素D在大鼠巨噬细胞抗血清中的添加
趋化蛋白-1(MCP-1)对条件培养液的影响
抑制单核细胞趋化,提示MCP-1可能在一定程度上是
对观察到的趋化活动负责。我们确认了
大鼠胰岛单核细胞趋化蛋白-1mRNA的原位杂交合成
(-istlet的细胞似乎从初级开始就是MCP-1的来源
(-)细胞和胰岛素瘤细胞系RINm5F表达MCP-1mRNA
IL-1激活。这些结果表明,胰腺(-)细胞
在IL-1激活时产生MCP-1,可能在
与自身免疫性糖尿病相关的胰岛素炎的进展。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('G KWON', 18)}}的其他基金
IL 1 INDUCED EXPRESSION OF MONOCYTE CHEMOATTRACTANT PROTEIN W/ TYPE I DIABETES
IL 1 诱导 I 型糖尿病单核细胞趋化蛋白的表达
- 批准号:
6665849 - 财政年份:2002
- 资助金额:
$ 0.6万 - 项目类别:
IL 1 INDUCED EXPRESSION OF MONOCYTE CHEMOATTRACTANT PROTEIN W/ TYPE I DIABETES
IL 1 诱导 I 型糖尿病单核细胞趋化蛋白的表达
- 批准号:
6486729 - 财政年份:2001
- 资助金额:
$ 0.6万 - 项目类别:
IL 1 INDUCED MONOCYTE CHEMOATTRACTANT PROTEIN: RAT ISLETS IN INSULITIS TYPE I DM
IL 1 诱导的单核细胞趋化蛋白:I 型胰岛素炎中的大鼠胰岛
- 批准号:
6118547 - 财政年份:1998
- 资助金额:
$ 0.6万 - 项目类别:
PROTEASOME COMPLEX IN IL 1: NITRIC OXIDE & PROSTAGLANDIN BY RAT ISLETS & B CELLS
IL 1 中的蛋白酶体复合物:一氧化氮
- 批准号:
6118546 - 财政年份:1998
- 资助金额:
$ 0.6万 - 项目类别:
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