INFRARED WAVE LENGTH MATRIX-ASSISTED LASER DESORPTION MASS SPECTROMETRY

红外波长矩阵辅助激光解吸质谱法

• 批准号: 6307547
• 负责人: SHUFANG NUI
• 金额: $ 0.82万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6307547
• 关键词: biomedical resource; proteins; spectrometry; technology /technique

MALDI-MS using UV wavelength laser irradiation has become a widely adopted technique for biological applications that require MW determination of peptides and proteins. IR wavelength laser irradiation has been much less used for MALDI-MS applications. One reason for the small amount of research in IR MALDI has been the lack of a convenient commercial IR-wavelength laser that produces pulses of short duration. All studies to date carried out using Er(YAG) lasers with a fixed wavelength of 2.94 prn and pulse duration in the range 100 - 200 ns. The recent commercial availability of optical parametric oscillators (OPO) that allow ready tuning of the laser wavelength in the range 0.7 - 4.0 prn and intense pulses of irradiation with duration - 6 ns has made it attractive for us to plan a thorough reinvestigation of IR MALDI. This investigation of IR MALDI is being undertaken using a Surelite 11 Nd(YAG) pump laser in combination with a Mirage 3000 OPO (Continuum, Santa Clara, CA.). We have successfully coupled the output of the OPO to our second custom built MALDI-TOF instrument and have carried out an extensive program of research with the new instrument. We have made a number of interesting findings that are reported in a paper in press (Niu et al, J. Am. Soc. Mass Spectrom.). These findings are of potential value both from a practical and a fundamental point of view (for obtaining an understanding of the MALD-MS process).

利用紫外光激光照射的MALDI-MS已经成为一种广泛的 采用的技术适用于需要MW的生物应用 多肽和蛋白质的测定。红外波长激光器 辐照在MALDI-MS应用中的使用要少得多。一 IR-MALDI研究较少的原因是缺乏 一种方便的商用红外波长激光器，它产生的脉冲 持续时间短。到目前为止，所有的研究都是使用Er(YAG)激光进行的 具有2.94prn的固定波长和在范围内的脉冲宽度 100-200纳秒。最近可用于商业用途的光纤 允许随时调谐激光器的参数振荡器(OPO) 0.7-4.0Prn范围内的波长和 持续时间为-6 ns的辐射使我们有兴趣计划 对IR MALDI的彻底重新调查。关于信息检索的这一研究 MALDI是使用Surelite 11 Nd(YAG)泵浦激光器进行的 与幻影3000 OPO(加利福尼亚州圣克拉拉的Continuum)相结合。我们 我已经成功地将OPO的输出连接到我们的第二个客户 建立了MALDI-TOF仪器，并开展了广泛的项目 使用新仪器进行研究。我们已经做了一些 在出版社的一篇论文中报告的有趣的发现(Niu等人， J.AmSoC。质谱学)。这些发现具有潜在的价值。 从实际和基本的角度来看(为了获得 对MALD-MS过程的了解)。