REGULATION OF THE MEIOTIC CELL CYCLE

描述：在有丝分裂周期中，只有一轮DNA复制 之后是染色体分离阶段，在此期间复制的基因 物质在子细胞之间被分割。减数分裂是一种特殊的 一种细胞周期，其中一个DNA复制阶段在连续两个阶段之前 染色体分离阶段。减数分裂特异的染色体调控因子 分离很可能作用于有丝分裂和减数分裂的共同机制。 染色体分离导致减数分裂染色体分离 程序。一种专门的泛素依赖的蛋白质分解机制--APC (后期促进复合体)，是染色体分离的关键调节因子 在有丝分裂期间。在酿酒酵母中，等电点已经表明APC 调节减数分裂重组，这对第一次减数分裂至关重要 染色体分离阶段。Spo13蛋白是一种减数分裂特异性调节蛋白 染色体分离的可能性。 本提案的目标是(1)确定APC在以下方面的作用 减数分裂重组，(2)确定Spo13如何控制减数分裂染色体 分离和(3)鉴定调控减数分裂染色体的新基因 种族隔离。为了表征APC在减数分裂重组中的作用， 重组的遗传和分子分析将被用来确定 重组过程中需要APC的步骤。生化和遗传学 然后，将使用方法来确定APC目标(S)，其破坏是 进行减数分裂重组所必需的。两种方法将是 用来鉴定减数分裂特异的染色体分离调节因子。第一, 已知的调节减数分裂染色体分离的Spo13将是 研究以确定它如何规范这一进程，并确定其目标。 其次，基因筛查将被用来识别新的调控基因 减数分裂，但不是有丝分裂染色体分离。

DESCRIPTION: During the mitotic cycle a single round of DNA replication is followed by a chromosome segregation phase, during which the duplicated genetic material is partitioned between the daughter cells. Meiosis is a specialized cell cycle in which a single DNA replication phase precedes two consecutive chromosome segregation phases. Meiosis-specific regulators of chromosome segregation are likely to act on the machinery common to mitotic and meiotic chromosome segregation to bring about the meiotic chromosome segregation program. A specialized ubiquitin-dependent proteolysis machinery, the APC (anaphase promoting complex), is a key regulator of chromosome segregation during mitosis. In Saccharomyces cerevisiae, the Pi has shown that the APC regulates meiotic recombination, which is critical for the first meiotic chromosome segregation phase. The protein Spo13 is a meiosis-specific regulator of chromosome segregation. The goals of this proposal are (1) to determine the role of the APC during meiotic recombination, (2) to determine how Spo13 controls meiotic chromosome segregation and (3) to identify new genes that regulate meiotic chromosome segregation. To characterize the role of the APC during meiotic recombination, genetic and molecular assays for recombination will be used to determine the step during recombination at which the APC is required. Biochemical and genetic approaches will then be used to identify APC target(s) whose destruction is required for the execution of meiotic recombination. Two approaches will be taken to identify meiosis-specific regulators of chromosome segregation. First, Spo13, which is known to regulate meiotic chromosome segregation will be studied to determine how it regulates this process and to identify its targets. Second, a genetic screen will be employed to identify new genes that regulate meiotic but not mitotic chromosome segregation.