REGULATION OF THE MEIOTIC CELL CYCLE

减数分裂细胞周期的调节

• 批准号: 6520358
• 负责人: ANGELIKA B AMON
• 金额: $ 22.34万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6520358
• 关键词: Saccharomyces cerevisiae; chromosome movement; fungal genetics; fungal proteins; genetic recombination; meiosis; nucleoproteins; protein structure function

DESCRIPTION: During the mitotic cycle a single round of DNA replication is followed by a chromosome segregation phase, during which the duplicated genetic material is partitioned between the daughter cells. Meiosis is a specialized cell cycle in which a single DNA replication phase precedes two consecutive chromosome segregation phases. Meiosis-specific regulators of chromosome segregation are likely to act on the machinery common to mitotic and meiotic chromosome segregation to bring about the meiotic chromosome segregation program. A specialized ubiquitin-dependent proteolysis machinery, the APC (anaphase promoting complex), is a key regulator of chromosome segregation during mitosis. In Saccharomyces cerevisiae, the Pi has shown that the APC regulates meiotic recombination, which is critical for the first meiotic chromosome segregation phase. The protein Spo13 is a meiosis-specific regulator of chromosome segregation. The goals of this proposal are (1) to determine the role of the APC during meiotic recombination, (2) to determine how Spo13 controls meiotic chromosome segregation and (3) to identify new genes that regulate meiotic chromosome segregation. To characterize the role of the APC during meiotic recombination, genetic and molecular assays for recombination will be used to determine the step during recombination at which the APC is required. Biochemical and genetic approaches will then be used to identify APC target(s) whose destruction is required for the execution of meiotic recombination. Two approaches will be taken to identify meiosis-specific regulators of chromosome segregation. First, Spo13, which is known to regulate meiotic chromosome segregation will be studied to determine how it regulates this process and to identify its targets. Second, a genetic screen will be employed to identify new genes that regulate meiotic but not mitotic chromosome segregation.

描述：在有丝分裂周期中，DNA复制是单轮的