CLONING OF THE 6Q27 TUMOR SUPPRESSOR GENE
2027 年 6 季度肿瘤抑制基因的克隆
基本信息
- 批准号:6300584
- 负责人:
- 金额:$ 25.09万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2000
- 资助国家:美国
- 起止时间:2000-05-01 至 2001-04-30
- 项目状态:已结题
- 来源:
- 关键词:breast neoplasms cell differentiation cell line cell proliferation chromosome deletion chromosomes gene expression gene mutation gene targeting genetic mapping genetic recombination genetically modified animals human tissue laboratory mouse loss of heterozygosity molecular cloning neoplasm /cancer genetics neoplastic process ovary neoplasms structural genes tumor suppressor genes
项目摘要
Deletions of chromosome 6q27 are associated at high frequency with various
common tumor types including non-Hodgkin lymphoma (NHL;>20%), ovarian
carcinoma (>70%), and breast carcinoma (approximately 50%). Loss of
heterozygosity (LOH), radiation-hybrid mapping and cloning studies have
identified a minimal region of loss within 6q27 common to all NHL cases
and, within this region, a 60kb subregion containing the minimal regions
of loss in breast and ovarian carcinoma. These associations suggest that
6q27 may contain a tumor suppressor gene (DOBL; deleted in ovarian, breast
carcinoma and lymphoma) altered in multiple cancer types. The goal of this
project is to identify the DOBL gene and to elucidate the role of its
alterations in tumorigenesis. The following specific aims will be pursued:
1) Identification of the DOBL gene by mutation analysis of genes mapping
within the 60 kb minimal deletion region in breast carcinoma cases
carrying heterozygous deletions or wild-type 6q27 regions. 2. Screening
ovarian carcinoma, NHL and other tumors for DOBL mutations in order to
determine whether the same suppressor gene is involved in 6q27 deletions
in different tumor types. 3. Characterization of the structure and pattern
of expression of the DOBL gene product by determining it structural
organization and homology to known proteins, its subcellular localization
and its pattern of expression during development, tissue proliferation and
differentiation. 4. Characterization of the biological function of the
DOBL gene, including the analysis of its tumor suppressor activity in
transfected cell lines in vitro, and its disruption in the mouse germ-line
to determine its role on tissue-development and tumorigenesis in vivo.
染色体 6q27 的缺失与多种疾病高频率相关。
常见肿瘤类型包括非霍奇金淋巴瘤(NHL;>20%)、卵巢癌
癌(>70%)和乳腺癌(约 50%)。损失
杂合性 (LOH)、辐射杂交作图和克隆研究
确定了所有 NHL 病例共有的 6q27 内的最小缺失区域
并且,在此区域内,包含最小区域的 60kb 子区域
乳腺癌和卵巢癌的损失。这些协会表明
6q27 可能含有抑癌基因(DOBL;在卵巢、乳腺癌中缺失)
癌和淋巴瘤)在多种癌症类型中发生改变。此举的目标
该项目的目的是鉴定 DOBL 基因并阐明其作用
肿瘤发生的改变。将追求以下具体目标:
1) 通过基因图谱突变分析鉴定DOBL基因
乳腺癌病例中 60 kb 最小缺失区域内
携带杂合缺失或野生型 6q27 区域。 2. 筛选
卵巢癌、NHL 和其他肿瘤进行 DOBL 突变,以便
确定 6q27 缺失是否涉及相同的抑制基因
在不同的肿瘤类型中。 3.结构和图案的表征
通过确定 DOBL 基因产物的结构来评估其表达
与已知蛋白质的组织和同源性、其亚细胞定位
及其在发育、组织增殖和发育过程中的表达模式
差异化。 4. 生物学功能的表征
DOBL基因,包括其抑癌活性分析
体外转染细胞系及其对小鼠种系的破坏
以确定其在体内组织发育和肿瘤发生中的作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Riccardo Dalla-Favera其他文献
Riccardo Dalla-Favera的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Riccardo Dalla-Favera', 18)}}的其他基金
From pathogenesis to new therapeutic targets in diffuse large B cell lymphoma
弥漫性大B细胞淋巴瘤从发病机制到新的治疗靶点
- 批准号:
10737214 - 财政年份:2023
- 资助金额:
$ 25.09万 - 项目类别:
From pathogenesis to new therapeutic targets in Diffuse Large B cell Lymphoma
弥漫性大 B 细胞淋巴瘤从发病机制到新的治疗靶点
- 批准号:
10453790 - 财政年份:2016
- 资助金额:
$ 25.09万 - 项目类别:
From pathogenesis to new therapeutic targets in Diffuse Large B cell Lymphoma
弥漫性大 B 细胞淋巴瘤从发病机制到新的治疗靶点
- 批准号:
9528531 - 财政年份:2016
- 资助金额:
$ 25.09万 - 项目类别:
From pathogenesis to new therapeutic targets in Diffuse Large B cell Lymphoma
弥漫性大 B 细胞淋巴瘤从发病机制到新的治疗靶点
- 批准号:
9186876 - 财政年份:2016
- 资助金额:
$ 25.09万 - 项目类别:
From pathogenesis to new therapeutic targets in Diffuse Large B cell Lymphoma
弥漫性大 B 细胞淋巴瘤从发病机制到新的治疗靶点
- 批准号:
9977975 - 财政年份:2016
- 资助金额:
$ 25.09万 - 项目类别:
From pathogenesis to new therapeutic targets in Diffuse Large B cell Lymphoma
弥漫性大 B 细胞淋巴瘤从发病机制到新的治疗靶点
- 批准号:
10224858 - 财政年份:2016
- 资助金额:
$ 25.09万 - 项目类别:
From pathogenesis to new therapeutic targets in Diffuse Large B cell Lymphoma
弥漫性大 B 细胞淋巴瘤从发病机制到新的治疗靶点
- 批准号:
9326271 - 财政年份:2016
- 资助金额:
$ 25.09万 - 项目类别:
Role of MEF2B mutations in lymphomagenesis
MEF2B 突变在淋巴瘤发生中的作用
- 批准号:
8697703 - 财政年份:2014
- 资助金额:
$ 25.09万 - 项目类别:
Role of MEF2B mutations in lymphomagenesis
MEF2B 突变在淋巴瘤发生中的作用
- 批准号:
8849397 - 财政年份:2014
- 资助金额:
$ 25.09万 - 项目类别:
相似海外基金
Hedgehog signalling in T-cell differentiation and function
T 细胞分化和功能中的 Hedgehog 信号传导
- 批准号:
BB/Y003454/1 - 财政年份:2024
- 资助金额:
$ 25.09万 - 项目类别:
Research Grant
Comparative single-cell analysis of disease-derived stem cells to identify the cell fate defect on the cell differentiation trajectory
对疾病来源的干细胞进行比较单细胞分析,以确定细胞分化轨迹上的细胞命运缺陷
- 批准号:
23H02466 - 财政年份:2023
- 资助金额:
$ 25.09万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The role of cell differentiation in colorectal cancer progression
细胞分化在结直肠癌进展中的作用
- 批准号:
23K06661 - 财政年份:2023
- 资助金额:
$ 25.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Dissecting the role of hypoxia in T cell differentiation in cancer
剖析缺氧在癌症 T 细胞分化中的作用
- 批准号:
10578000 - 财政年份:2023
- 资助金额:
$ 25.09万 - 项目类别:
Mechanisms mediating human enteroendocrine cell differentiation and function
介导人肠内分泌细胞分化和功能的机制
- 批准号:
10739834 - 财政年份:2023
- 资助金额:
$ 25.09万 - 项目类别:
TOX-driven CD8 T cell differentiation and dysfunction in tumors
TOX驱动的肿瘤中CD8 T细胞分化和功能障碍
- 批准号:
10586679 - 财政年份:2023
- 资助金额:
$ 25.09万 - 项目类别:
Elucidation of molecular mechanisms of immune cell differentiation of a novel Rab protein in hematopoietic stem cells
阐明造血干细胞中新型Rab蛋白免疫细胞分化的分子机制
- 批准号:
23K16122 - 财政年份:2023
- 资助金额:
$ 25.09万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
New strategies in cell replacement therapies for diabetes: role of USP7 in iPSC and adult organoids beta cell differentiation
糖尿病细胞替代疗法的新策略:USP7 在 iPSC 和成体类器官 β 细胞分化中的作用
- 批准号:
MR/X01813X/1 - 财政年份:2023
- 资助金额:
$ 25.09万 - 项目类别:
Research Grant
Role of alveolar fibroblasts in extracellular matrix organization and alveolar type 1 cell differentiation
肺泡成纤维细胞在细胞外基质组织和肺泡1型细胞分化中的作用
- 批准号:
10731854 - 财政年份:2023
- 资助金额:
$ 25.09万 - 项目类别:
Exhaustive Identification of Essential Genes for Human Taste Cell Differentiation ~Development of a Method for Inducing Differentiation of Taste Buds from ES/iPS Cells~
彻底鉴定人类味觉细胞分化必需基因~开发诱导ES/iPS细胞味蕾分化的方法~
- 批准号:
23K09214 - 财政年份:2023
- 资助金额:
$ 25.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)