CHARGE COUPLED DEVICE INSTRUMENTATION AND INFORMATICS

电荷耦合器件仪器仪表和信息学

• 批准号: 6300534
• 负责人: MITCHELL D EGGERS
• 金额: $ 25.29万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-14 至 2002-01-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6300534
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; charge coupled device camera; chelating agents; computer assisted diagnosis; computer human interaction; computer program /software; computer system design /evaluation; digital imaging; fluorescence; fluorescent dye /probe; image processing; information systems; lung neoplasms; messenger RNA; neoplasm /cancer diagnosis; statistics /biometry

The overall objective of the instrumentation project is the prototype development of user-friendly detection/imaging hardware with informatics software to support high throughput mRNA analyses of lung tissue samples in a microarray microtiter-based format. The custom instrumentation to be developed for the Program Project is based on the proximal CCD detection/imaging technology established at Genometrix under both federal (DOC/ATP, NIH, NASA) and private (venture capital, corporate partners) funding. The functional specifications for the instrumentation developed for the Program Project will facilitate high sensitivity for chemiluminescent and fluorescent labeling, aray multiplexing, high throughput, automated quantitation, and information processing. Specifically, the instrumentation workstation will accommodate both the fluorescent and chemiluminescent labeling formats proposed in Project 1. Several motifs utilizing Eu/+3 chelators will be accommodated including linkage to beads in a sandwich assay, covalent attachment to mRNA, and simple sandwich assays using enzyme linked fluorescence. The proposed proximal CCD detection system will be automatically time gated to reduce background fluorescence. The proposed proximal CCD detection system will be automatically time gated to reduce background fluorescence, thereby taking full advantage of the long emission lifetimes characteristics of the chelating compounds. Also dioxitane chemiluminescent labeling will be employed wherein both linkage to detector probes and to beads in a sandwich assay will be accommodated. Finally, the informatics component of the workstation involves converting the pattern of array binding patterns into relative mRNA concentrations. Approaches to be investigated for this deconvolution problem include conventional statistical correlation analysis as well as neural networks and multicomponent maximum entropy methods. Following prototype development, the above instrumentation will be made available to scientists in Core A for use by all Program Project members. Also the workstations will be built in close collaboration with Program Project members, (especially members from Project 1, Core A and B) to ensure high compatibility with the multiparameter lung tissue mRNA analyses.

仪器项目的总体目标是原型 利用信息学开发用户友好的检测/成像硬件 支持肺组织样本高通量mRNA分析的软件 以微阵列微量滴定为基础的形式。 自定义检测， 为该计划项目开发的是基于近端CCD的 在基因组学研究所建立的检测/成像技术， 联邦（DOC/ATP、NIH、NASA）和私人（风险投资、公司 合作伙伴）供资。 的功能规格 为该计划项目开发的仪器将促进高 荧光标记灵敏度 多路复用、高通量、自动定量和信息 处理. 具体而言，仪器工作站将同时容纳 项目中提出的荧光和荧光标记格式 1. 将容纳几个利用Eu/+3螯合剂的图案 包括在夹心测定中与珠的连接、共价连接到 mRNA和使用酶联荧光的简单夹心测定。 的 建议的近端CCD检测系统将自动进行时间选通 以减少背景荧光。 建议的近端CCD检测 系统将自动定时门控，以减少背景 荧光，从而充分利用长发射 螯合化合物的寿命特性。 还有二氧杂环丁烷 将采用荧光标记，其中与荧光标记的连接 将容纳检测器探针和夹心测定中的珠。 最后，工作站的信息学部分包括 将阵列结合模式的模式转换成相对mRNA， 浓度的 这种去卷积的研究方法 问题还包括常规统计相关分析 如神经网络和多分量最大熵方法。 在原型开发之后，将制造上述仪器。 供核心A的科学家使用，供所有计划项目 成员 此外，工作站将建立在密切合作 与计划项目成员（特别是来自项目1，核心 A和B）确保与多参数肺的高度兼容性 组织mRNA分析。