CHARGE COUPLED DEVICE INSTRUMENTATION AND INFORMATICS

仪器项目的总体目标是原型 利用信息学开发用户友好的检测/成像硬件 支持高通量肺组织样本信使核糖核酸分析的软件 以微阵列微滴度为基础的形式。自定义指令插入到 基于近端电荷耦合器件的BE方案开发 在Genometrix建立的检测/成像技术 联邦(DOC/ATP、NIH、NASA)和私人(风险投资、企业 合作伙伴)提供资金。的功能规格说明 为计划项目开发的仪器将促进高 化学发光和荧光标记的灵敏度 多路传输、高吞吐量、自动量化和信息 正在处理。 具体地说，仪表工作站将同时容纳 项目中建议的荧光和化学发光标记格式 1.将容纳几个使用Eu/3螯合剂的基序 包括在三明治化验中连接到珠子、共价连接到 用酶联荧光法进行简单的夹心法检测。这个 拟议的近端CCD检测系统将自动定时选通 以减少背景荧光。建议的近端电荷耦合器件检测 系统将自动设置时间门控以减少背景 荧光，从而充分利用长发射 螯合化合物的寿命特征。也叫二氧六环 将使用化学发光标记，其中两个链接都 将容纳夹心分析中的检测器探头和TO珠子。 最后，工作站的信息学部分涉及 将数组结合模式转换为相对mRNA 浓度。这种反卷积需要研究的方法 问题还包括传统统计相关分析 如神经网络和多分量最大熵方法。 在样机开发后，将进行上述仪器的研制 可供核心A的科学家使用，供所有计划项目使用 会员。此外，这些工作站将在密切合作中建造 具有计划项目成员(特别是来自项目1、核心的成员 A和B)以确保与多参数肺的高兼容性 组织信使核糖核酸分析。

The overall objective of the instrumentation project is the prototype development of user-friendly detection/imaging hardware with informatics software to support high throughput mRNA analyses of lung tissue samples in a microarray microtiter-based format. The custom instrumentation to be developed for the Program Project is based on the proximal CCD detection/imaging technology established at Genometrix under both federal (DOC/ATP, NIH, NASA) and private (venture capital, corporate partners) funding. The functional specifications for the instrumentation developed for the Program Project will facilitate high sensitivity for chemiluminescent and fluorescent labeling, aray multiplexing, high throughput, automated quantitation, and information processing. Specifically, the instrumentation workstation will accommodate both the fluorescent and chemiluminescent labeling formats proposed in Project 1. Several motifs utilizing Eu/+3 chelators will be accommodated including linkage to beads in a sandwich assay, covalent attachment to mRNA, and simple sandwich assays using enzyme linked fluorescence. The proposed proximal CCD detection system will be automatically time gated to reduce background fluorescence. The proposed proximal CCD detection system will be automatically time gated to reduce background fluorescence, thereby taking full advantage of the long emission lifetimes characteristics of the chelating compounds. Also dioxitane chemiluminescent labeling will be employed wherein both linkage to detector probes and to beads in a sandwich assay will be accommodated. Finally, the informatics component of the workstation involves converting the pattern of array binding patterns into relative mRNA concentrations. Approaches to be investigated for this deconvolution problem include conventional statistical correlation analysis as well as neural networks and multicomponent maximum entropy methods. Following prototype development, the above instrumentation will be made available to scientists in Core A for use by all Program Project members. Also the workstations will be built in close collaboration with Program Project members, (especially members from Project 1, Core A and B) to ensure high compatibility with the multiparameter lung tissue mRNA analyses.