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CORE--TISSUE CULTURE, VIRUS AND ANTIBODY FACILITY

CORE--TISSUE CULTURE, VIRUS AND ANTIBODY FACILITY
核心——组织培养、病毒和抗体设施
批准号：
6300576
负责人：
STEPHEN Paine GOFF
金额：
$11.73万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
项目状态：
已结题
起止时间：
2000-01-31 至 2000-12-31
来源：
https://reporter.nih.gov/project-details/6300576
关键词：
Abelson leukemia virus biomedical facility bone marrow cell transformation cryopreservation fibroblasts laboratory mouse microorganism resource /registry molecular cloning monoclonal antibody tissue /cell culture tissue resource /registry transfection

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This core will provide tissue culture-derived reagents for all four projects. The core will make and titer stocks of mutant and wt forms of v-abl and bcr-abl viruses. The core will also use these viruses to transform murine bone marrow cells and fibroblast ell lines in vitro and to infect mice in vivo. The core will also obtain from different projects wt and mutant forms of Jak and Abi-1 proteins and will generate stably transfected lines expressing these proteins in an inducible manner. The core will generate monoclonal antibodies and will provide routine cell culture and freezing for all of the projects.
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Role of heme oxygenase 2 in trafficking and regulation of myristoylated proteins
Microtubule networks and virus trafficking
Microtubule networks and virus trafficking
Regulatuion of HIV-1 Gene Expression in Latency by YY1, RuvB2, and ZAP
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