CORE--TISSUE CULTURE, VIRUS AND ANTIBODY FACILITY

核心——组织培养、病毒和抗体设施

• 批准号: 6563885
• 负责人: STEPHEN Paine GOFF
• 金额: $ 22.84万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-14 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563885
• 关键词: Abelson leukemia virus; biomedical facility; bone marrow; cell transformation; cryopreservation; fibroblasts; laboratory mouse; microorganism resource /registry; molecular cloning; monoclonal antibody; tissue /cell culture; tissue resource /registry; transfection

This core will provide tissue culture-derived reagents for all four projects. The core will make and titer stocks of mutant and wt forms of v-abl and bcr-abl viruses. The core will also use these viruses to transform murine bone marrow cells and fibroblast ell lines in vitro and to infect mice in vivo. The core will also obtain from different projects wt and mutant forms of Jak and Abi-1 proteins and will generate stably transfected lines expressing these proteins in an inducible manner. The core will generate monoclonal antibodies and will provide routine cell culture and freezing for all of the projects.

这个核心将为所有四个人提供组织培养衍生试剂 项目。核心将制造和滴定突变形式和wt形式的股票 V-ABL和BCR-ABL病毒。核心还将使用这些病毒来 体外转化小鼠骨髓细胞和成纤维细胞系 在体内感染小鼠。核心还将从不同的 项目wt和突变形式的Jak和Abi-1蛋白，并将产生 在诱导物中稳定表达这些蛋白的细胞系 举止。核心将产生单抗，并将提供 所有项目的常规细胞培养和冷冻。