FLUORESCENCE SATURATION IN 3 LEVEL SYSTEMS IN FLUORESCENCE CORRELATION SPECT

荧光相关光谱中三级系统的荧光饱和度

• 批准号: 6349392
• 负责人: AHMED A HEIKAL
• 金额: $ 1.06万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349392
• 关键词: bioengineering /biomedical engineering; biological products; biomedical equipment development; biomedical resource; biotechnology; lasers; technology /technique

We have been evaluating the data we have collected for our action spectrum of DNA synthesis inhibition to determine the excitation order, n, using the general dose-response equation, , where S is the normalized survival and the dose, D, is defined as Int. (The irradiance, I, is in photons/s((m2, and n is the number of photons required for a single excitation event, or the excitation order.) Preliminary analysis suggests that the observed damage is arising from different absorbing species at different wavelengths -- at 700 nm damage appears to be from one-photon events and at 740 nm it appears to be from two-photon events. Further studies are underway to confirm this observation.

我们一直在评估为我们的行动收集的数据 DNA合成抑制光谱，以确定激发 阶，n，使用一般剂量反应方程，其中S是 标准化生存期，剂量D定义为Int.（ 辐照度I的单位为光子/s（（m2，n为光子数 单个激励事件或激励顺序所需）。 初步分析表明，所观察到的损害是由于 不同波长下的不同吸收物质--在700 nm处 损伤似乎来自单光子事件，在740 nm处， 来自于双光子事件 进一步的研究正在进行中， 这个观察。