TWO PHOTON FLUORESCENCE CORRELATION SPECT OF RHODAMINE GREEN IN SOLUTION

溶液中罗丹明绿的双光子荧光相关谱

• 批准号: 6349396
• 负责人: AHMED A HEIKAL
• 金额: $ 1.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349396
• 关键词: bioengineering /biomedical engineering; biological products; biomedical equipment development; biomedical resource; lasers; technology /technique

In one- and multi-photon fluorescence microscopy, photobleaching of the fluorescent markers under intense light is a limiting factor. A fluorophore under an intense illumination can undergo photobleaching through either unimolecular or bimolecular reaction in which the photon energy and the enviroment play a major rule. Reliable techniques for photobleaching quantum yield (?b) measurements are crucial for fundamental understanding of the mechanisms and pathways involved in bleaching dynamics. On a single molecule level, fluorescence correlation spectroscopy (FCS) is a powerfull technique for diffusion as well as triplet-state characterization. Theoritical analysis has been carried out to extend the use of FCS for photobleaching studies. In those analysis, triplet- and first excited singlet-state are considered as possible photobleaching pathways. Enviromental effects, such as singlet oxygen and external heavy-atom effects, were also discussed.

在单光子和多光子荧光显微镜中， 荧光标记物在强光下的降解是一个限制因素。 荧光团在强光照下会发生光漂白 通过单分子或双分子反应， 光子能量和环境起主要作用。 可靠 技术的光漂白量子产率（？B）测量值是 对于从根本上理解这些机制和途径至关重要 参与漂白动力学。 在单分子水平上， 荧光相关光谱（FCS）是一种强有力的技术 用于扩散以及三重态表征。 的理论 已经进行了分析，以扩大FCS的使用范围， 光漂白研究。 在这些分析中，三重态和第一激发态 单线态被认为是可能的光漂白途径。 环境效应，如单线态氧和外部重原子 的影响，也进行了讨论。