FIBER OPTIC MULTIPHOTON EXCITATION OF TISSUE AUTOFLUORESCENCE: DIS TRANSFORM

特定蛋白质的膜定位和扩散 对蛋白质和细胞的 功能协调发展的 脂质修饰通常被细胞用来 靶向蛋白质到质膜的内小叶; 这种酰化蛋白质的分布和横向移动性是 我们实验的重点。 将EGFP构建体转染到RBL中， 细胞和它们的扩散以及膜之间的分配 并通过FCS分析和表征胞质标记。

The membrane localisation and diffusion of particular proteins within a cell is critical to the protein's and the the cell's functions. Lipid modifications are commonly employed by the cell to target proteins to the inner leaflet of the plasma membrane; the distribution and lateral mobility of such acylated proteins is the focus of our experiments. EGFP constructs were transfected into RBL cells and their diffusion as well as the partition between membrane and cytosolic labeling analyzed and characterized by FCS.