PHOTODYNAMIC SENSITIZERS ON CELL AUTOFLUORESCENCE: MULTICELL TUMOR SPHEROIDS

荧光标记分子的聚集过程可以是 便于FCS研究。相关幅度是敏感的 测量中同时存在的分子的数量 体积元素，即显微镜物镜的焦点。 此外，扩散特性在以下过程中会发生变化 聚集从而影响分子在体系中的停留时间 照明区域。我们研究了聚集的时间进程 涉及EGFP标记的膜蛋白与外源蛋白连接的过程 受体和添加抗原对基因工程的刺激 RBL细胞。

Aggregation processes of fluorescent labeled molecules can be conveniently studied by FCS. The correlation amplitude is sensitive to the number of molecules contemporarily present in the measurement volume element, i.e. the focal spot of a microscope objective. Moreover, the diffusion characteristics are subject to change during aggregation thereby affecting the molecular residence time in the illuminated area. We investigated the time course of aggregation processes involving EGFP labeled membrane proteins linked to external receptors and stimulated by adding antigen on genetically engineered RBL cells.