OVEREXPRESSION OF NERVE GROWTH FACTOR

神经生长因子过度表达

• 批准号: 6165482
• 负责人: BRIAN M DAVIS
• 金额: $ 26.65万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6165482
• 关键词: afferent nerve; animal genetic material tag; developmental genetics; developmental neurobiology; gene expression; genetically modified animals; laboratory mouse; nerve injury; neurogenesis; neurogenetics; neurotrophic factors; neutralizing antibody; peripheral nervous system; ventral column; wound healing

DESCRIPTION: Neurotrophins are known to control not only neuronal survival, but may modulate phenotypic differentiation and adult neuropathic pain. In transgenic mice overexpressing NT-3 or NGF, changes are noted in sensory neuron number, projections and pain threshold. The collaborating lab has the ability to determine the "Comprehensive phenotype" of adult sensory neurons, using intracellular recording and labeling. These techniques will be used to examine individual sensory neurons of control and transgenic mice to assess if neurotrophin overexpression alters the phenotype (aims 1 and 2). It is also hypothesized that neuropathic pain recapitulates neurotrophin dependent processes. In aim 3, the phenotype of neurons responsible for neuropathic pain will be characterized, and antineurotrophin antisera will be used to block formation of the sympathetic/sensory neuron connections thought to mediate neuropathic pain.

描述：已知神经营养因子不仅控制神经元的存活， 但可能调节表型分化和成人神经性疼痛。 在 过表达NT-3或NGF的转基因小鼠，在感觉神经元中观察到变化。 神经元数量、投射和痛阈。 合作实验室已 确定成人感觉神经元“综合表型”的能力 神经元，使用细胞内记录和标记。 这些技术将 用于检查对照和转基因小鼠的单个感觉神经元 为了评估神经营养因子过表达是否改变了表型（aims 1和 2）。 也有人假设，神经性疼痛重演 神经营养因子依赖过程。 在目标3中，神经元的表型 负责神经病理性疼痛的特征，和抗神经营养因子 抗血清将用于阻断交感/感觉神经元的形成 被认为介导神经性疼痛的连接。