Core--Morphology and immunohistochemistry facility

形态学和免疫组织化学核心科学摘要了解糖尿病影响视网膜和肾脏内的哪些细胞类型对于有效的靶向治疗至关重要。特定蛋白质和细胞死亡标记物的细胞定位，以及对治疗对视网膜和肾脏结构的影响的量化分析，是当前提案中项目的组成部分。具体目标1：形态和免疫组织化学核心的目的是通过提供：a.组织收集和处理的技术支持，b.评估视网膜和肾脏组织病理学的专家协助，以及c.量化试剂和免疫组织化学的技术支持，帮助执行基于解剖学的实验。具体目标2：形态和免疫组织化学核心将通过提供项目所必需但超出调查人员现有能力的专家援助和特殊设施，促进和促进个别项目的研究。它还将提供质量控制，促进调查人员之间的互动，并在这些技术领域提供教学。

Morphology and Immunohistochemistry Core Scientific Abstract An understanding of which cell types within the retina and kidney are affected by diabetes is essential for effective treatment targeting. The cellular localization of specific proteins and cell death markers, and the quantitative analysis of effects of therapies on retinal and renal structure are integral parts of the projects in the current proposal. Specific Aim 1: The purpose of the Morphology and Immunohistochemistry Core is to aid in the execution of anatomically- based experiments by providing: a. technical support for tissue collection and processing, b. expert assistance in evaluation of retinal and renal histopathology, and c. quantify reagents and technical support for immunohistochemistry. Specific Aim 2: The Morphology and Immunohistochemistry Core will facilitate and stimulate research in individual projects by providing expert assistance and special facilities essential for the projects but beyond the current capabilities of investigators. It will also provide quality control and facilitate interaction between investigators, and provide teaching in these technical areas.