Core--Morphology and immunohistochemistry facility

核心--形态学和免疫组化设施

• 批准号: 6504049
• 负责人: MARK A SMITH
• 金额: $ 9.46万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6504049
• 关键词: animal tissue; biomedical facility; clinical research; diabetes mellitus; histology; human tissue; immunocytochemistry

Morphology and Immunohistochemistry Core Scientific Abstract An understanding of which cell types within the retina and kidney are affected by diabetes is essential for effective treatment targeting. The cellular localization of specific proteins and cell death markers, and the quantitative analysis of effects of therapies on retinal and renal structure are integral parts of the projects in the current proposal. Specific Aim 1: The purpose of the Morphology and Immunohistochemistry Core is to aid in the execution of anatomically- based experiments by providing: a. technical support for tissue collection and processing, b. expert assistance in evaluation of retinal and renal histopathology, and c. quantify reagents and technical support for immunohistochemistry. Specific Aim 2: The Morphology and Immunohistochemistry Core will facilitate and stimulate research in individual projects by providing expert assistance and special facilities essential for the projects but beyond the current capabilities of investigators. It will also provide quality control and facilitate interaction between investigators, and provide teaching in these technical areas.

形态学和免疫组织化学核心科学摘要了解视网膜和肾脏中哪些细胞类型受糖尿病影响对于有效的治疗靶向至关重要。特定蛋白质和细胞死亡标志物的细胞定位以及治疗对视网膜和肾脏结构影响的定量分析是当前提案中项目的组成部分。具体目标1：形态学和免疫组织化学核心的目的是通过提供以下内容来帮助执行基于解剖学的实验：组织收集和处理技术支持，B.视网膜和肾组织病理学评估的专家协助，以及c.免疫组化定量试剂和技术支持。具体目标二：形态学和免疫组化核心将通过提供专家援助和项目所需的特殊设施，但超出了研究人员目前的能力，促进和刺激个别项目的研究。它还将提供质量控制，促进调查人员之间的互动，并提供这些技术领域的教学。