CORE--ANIMAL PHYSIOLOGY AND LIPID ANALYSIS

核心--动物生理学和脂质分析

• 批准号: 6301197
• 负责人: LAURA A WOOLLETT
• 金额: $ 16.43万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6301197
• 关键词: analytical chemistry; biomedical facility; dietary lipid; hamsters; human subject; laboratory mouse; lipid metabolism; nutrition related tag; steroid biosynthesis

This Core Unit has two primary functions. One function of this Core Unit is to analyze plasma, tissue, and intestinal lumen samples for lipid concentration and composition, and to size micelles and vesicles. This Core Unit will also function as a source of expertise in a variety of studies of mice, in vivo rates of sterol synthesis in hamsters and mice, and LDL uptake rates in hamsters. Due to the types of analyses being completed, this Core will serve all projects in a highly interactive and collaborative manner. The first function of this Core is to analyze lipid concentration and content of different types of samples generated from humans and animals. Plasma total and LDL cholesterol concentrations will be determined by standardized techniques. Postprandial samples from intestinal lumen of both animals and humans will be assayed for cholesterol solubility, micellar and vesicular size, bile acid composition and concentration, cholesterol concentration, phospholipid concentration and glyceride concentration. The second function of this Core utilizes the expertise of the lead and participating investigators. Cholesterol and triglyceride absorption, as determined by secretion of radiolabeled lipid into lymph, will be measured in this Core in mice lacking various digestive enzymes. In addition, this ore will study sterol balance across mice lacking the same digestive enzymes by measuring in vivo rates of synthesis in the liver, small intestine and remaining carcass. Finally, in vivo sterol synthesis rates and LDL-cholesterol uptake rates will be determined in hamsters fed diets similar to human if the various diets resulted in changes in sterol fractional synthetic rates, LDL-cholesterol levels or mRNA levels for HMG-CoA reductase or the LDL receptor.

该核心股有两个主要职能。该核心股的一项职能是 分析血浆组织和肠腔样本中的脂质 浓度和组成，以及大小胶束和囊泡。这 核心股还将作为各种领域的专门知识来源， 小鼠的研究，仓鼠和小鼠中甾醇合成的体内速率， 和仓鼠的LDL摄取率。由于分析的类型 完成后，该核心将以高度互动和 合作的方式。 该核心的第一个功能是分析脂质浓度， 从人类和动物中产生的不同类型样本的含量。 血浆总胆固醇和LDL胆固醇浓度将通过 标准化技术。来自肠腔的餐后样本 动物和人都将测定胆固醇溶解度， 胶束和囊泡大小，胆汁酸组成和浓度， 胆固醇浓度、磷脂浓度和甘油酯 浓度. 该核心的第二个功能是利用主管的专业知识， 参与调查者。胆固醇和甘油三酯的吸收， 通过放射性标记的脂质分泌到淋巴中来确定， 在缺乏各种消化酶的小鼠中进行测量。在 此外，这项研究还将研究缺乏 相同的消化酶，通过测量体内合成率， 肝脏、小肠和剩余的尸体。最后，体内固醇 合成速率和LDL-胆固醇摄取速率将在 仓鼠喂养的饮食与人类相似，如果各种饮食导致 固醇合成率、LDL-胆固醇水平或 HMG-CoA还原酶或LDL受体的mRNA水平。