CORE--ANIMAL PHYSIOLOGY AND LIPID ANALYSIS

核心--动物生理学和脂质分析

• 批准号: 6451086
• 负责人: LAURA A WOOLLETT
• 金额: $ 18.67万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6451086
• 关键词: analytical chemistry; biomedical facility; dietary lipid; hamsters; human subject; laboratory mouse; lipid metabolism; nutrition related tag; steroid biosynthesis

This Core Unit has two primary functions. One function of this Core Unit is to analyze plasma, tissue, and intestinal lumen samples for lipid concentration and composition, and to size micelles and vesicles. This Core Unit will also function as a source of expertise in a variety of studies of mice, in vivo rates of sterol synthesis in hamsters and mice, and LDL uptake rates in hamsters. Due to the types of analyses being completed, this Core will serve all projects in a highly interactive and collaborative manner. The first function of this Core is to analyze lipid concentration and content of different types of samples generated from humans and animals. Plasma total and LDL cholesterol concentrations will be determined by standardized techniques. Postprandial samples from intestinal lumen of both animals and humans will be assayed for cholesterol solubility, micellar and vesicular size, bile acid composition and concentration, cholesterol concentration, phospholipid concentration and glyceride concentration. The second function of this Core utilizes the expertise of the lead and participating investigators. Cholesterol and triglyceride absorption, as determined by secretion of radiolabeled lipid into lymph, will be measured in this Core in mice lacking various digestive enzymes. In addition, this ore will study sterol balance across mice lacking the same digestive enzymes by measuring in vivo rates of synthesis in the liver, small intestine and remaining carcass. Finally, in vivo sterol synthesis rates and LDL-cholesterol uptake rates will be determined in hamsters fed diets similar to human if the various diets resulted in changes in sterol fractional synthetic rates, LDL-cholesterol levels or mRNA levels for HMG-CoA reductase or the LDL receptor.

这个核心单元有两个主要功能。该核心单元的一项功能 是分析血浆、组织和肠腔样本中的脂质 浓度和组成，以及胶束和囊泡的大小。这 核心股还将作为各种专业知识的来源发挥作用 对小鼠的研究，仓鼠和小鼠体内甾醇合成的速度， 以及仓鼠的低密度脂蛋白摄取率。由于分析的类型是 完成后，这一核心将以高度互动和 合作的态度。 这个核心的第一个功能是分析脂质浓度和 来自人和动物的不同类型样本的含量。 血浆总胆固醇和低密度脂蛋白浓度将通过以下方式确定 标准化技术。餐后采集的肠腔样本 动物和人类都将被检测胆固醇的溶解度， 胶束和泡的大小，胆汁酸的组成和浓度， 胆固醇浓度、磷脂浓度和甘油三酯 集中精神。 此核心的第二个功能利用领导的专业知识和 参与调查的人员。胆固醇和甘油三酯的吸收，如 通过将放射性标记的脂质分泌到淋巴中来确定， 在缺乏各种消化酶的小鼠身上测量这一核心。在……里面 此外，这个矿石将研究缺乏类固醇的小鼠之间的类固醇平衡 相同的消化酶通过测量体内合成的速度 肝脏、小肠和剩余的身体。最后，体内的类固醇 合成率和低密度脂蛋白-胆固醇摄取率将在#年确定。 仓鼠喂食与人类相似的饮食，如果不同的饮食导致 甾醇合成率、低密度脂蛋白-胆固醇水平或 HMG-CoA还原酶或低密度脂蛋白受体的mRNA水平。