RESPONSE OF BONE TO INVADING BREAST ADENOCARCINOMA CELLS

我们的总体目标是了解溶骨性关节炎的病理生理学 骨对侵入的乳腺癌细胞的反应。 骨溶解 主要需要癌症介导的细胞失调， 破骨细胞谱系，但其他细胞类型也严重参与 例如基质细胞、成骨细胞和内皮细胞。 我们计划 确定一些与骨质溶解相关的调节因素， 由转移性腺癌细胞产生。 本研究的具体目的是分析 配体RANKL及其诱饵受体OPG通过骨细胞应答 在不同共培养条件下生长的乳腺癌细胞，和 确定乳腺癌细胞是否也表达RANKL或OPG， 无论是骨中诱导的还是乳腺癌分泌的蛋白酶 细胞表达RANKL或OPG。

Our general goal is to understand the pathophysiology osteolytic response of bone to invading breast adenocarcinoma cells. Osteolysis primarily requires cancer-mediated dysregulation of cells from the osteoclasi lineage, but other cells types are also critically involved e.g. stromal cells, osteoblasts, and endothelial cells. We plan to identify some of the regulatory factors relevant to osteolysis which are produced by metastatic adenocarcinoma cells. The specific aim of this study is to analyze the expression of the ligand RANKL and its decoy receptor OPG by bone cells in response to breast carcinoma cells grown under different co-culture conditions, and to determine whether breast carcinoma cells also express RANKL or OPG or whether proteinases induced in bone or secreted by breast carcinoma cells inactivate RANKL or OPG.