神经元分化的蛋白水解控制-猫眼课题宝

神经元分化的蛋白水解控制

批准号：

6337229

负责人：

James I Kim

金额：

$3.48万

依托单位：

UNIVERSITY OF CALIFORNIA LOS ANGELES

依托单位国家：

美国

项目类别：

财政年份：

2001

资助国家：

美国

项目状态：

未结题

起止时间：

2001-05-15 至

来源：

https://reporter.nih.gov/project-details/6337229

关键词：

cell differentiation gene deletion mutation gene expression gene targeting genetically modified animals in situ hybridization laboratory mouse molecular site neurogenesis northern blottings phenotype pluripotent stem cells postdoctoral investigator protease inhibitor proteolysis

中文摘要

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英文摘要

We have discovered a secreted, soluble protein that is able to inhibit primary neurogenesis in Xenopus embryos. This 394 amino acid protein contains three serine proteinase inhibitor domains of the Kunitz class. This novel protein has been named Trinein and was isolated during a differential screen for maternally-encoded mRNAs present in the Xenopus laevis egg. Strikingly, microinjection of Trinein mRNA into Xenopus embryos seems to have little effect on mesodermal (marked by Xbra, sonic hedgehog, chordin) and neural plate (marked by Sox-2) cell differentiation; however, differentiation of definitive mature neurons (positive for neurotubulin and neurogenin) is blocked in the neural plate (unpublished data). This proposal builds upon these functional data of Trinein to understand its function in mammalian neurogenesis.

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